大花蕙兰环斑病毒卫星RNA和缺陷干扰RNA的序列分析

Sequence analysis of cymbidium ringspot virus satellite and defective interfering RNAs.

作者信息

Rubino L, Burgyan J, Grieco F, Russo M

机构信息

Dipartimento di Patologia Vegetale, Università degli Studi, Bari, Italy.

出版信息

J Gen Virol. 1990 Aug;71 ( Pt 8):1655-60. doi: 10.1099/0022-1317-71-8-1655.

DOI:10.1099/0022-1317-71-8-1655

PMID:1697328

Abstract

The nucleotide sequences of cloned cDNA copies of satellite and defective interfering (DI) RNAs of cymbidium ringspot virus were determined. DI RNA is 499 nucleotides long and is composed of six stretches of sequence derived from CyRSV genomic RNA. Four of these stretches share common 5' sequences and the 5' and 3' ends are identical to those of genome RNA. Satellite RNA is 621 nucleotides long. In some regions the sequence is very similar (60% to 100% identical) to genomic RNA. A consensus sequence is proposed to be involved in the replication of genomic, DI and satellite RNAs.

摘要

测定了大花蕙兰环斑病毒卫星RNA和缺陷干扰（DI）RNA的克隆cDNA拷贝的核苷酸序列。DI RNA长499个核苷酸，由来自CyRSV基因组RNA的六个序列片段组成。其中四个片段具有共同的5'序列，其5'和3'末端与基因组RNA的相同。卫星RNA长621个核苷酸。在某些区域，该序列与基因组RNA非常相似（60%至100%相同）。提出了一个共有序列参与基因组、DI和卫星RNA的复制。