is a novel biomarker for poor prognosis in LUAD.

BACKGROUND

Approximately 80% of lung cancers are non-small cell lung cancers (NSCLC). Lung adenocarcinoma (LUAD) is the main subtype of NSCLC. The incidence and mortality of lung cancer are also increasing yearly. Myogenic differentiation family inhibitor () as a transcription factor, its role in lung cancer has not yet been clarified.

METHODS

LUAD data were downloaded from The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO), analyzed and plotted using the R language. Associations between Clinical information and expression were assessed using logistic regression analyses to explore the effects of on LUAD. Two sets of tissue microarrays (TMAs) further confirmed the overexpression of in LUAD and its impact on prognosis. In addition, we examined the correlation between and immune infiltration. To investigate the effect of on the biological behavior of LUAD tumor cells by GSEA and GO/KEGG analysis. The survival status and somatic mutational characteristics of patients according to levels were depicted and analyzed.

RESULTS

Expression of high in LUAD tissues analyzing TCGA dataset (0.001). Kaplan-Meier survival analysis indicated a poor prognosis for those patients with LUAD who had upregulated expression levels (0.001). This was also verified by two groups of TMAs (=0.024). Using logistic statistics analysis, was identified as an independent predictive factor and was associated with poor prognosis in LUAD (0.001, =0.021). Assessment of clinical characteristics, tumor mutation burden (TMB), and tumor microenvironment (TME) between high- and low-expression score groups showed lower TMB, richer immune cell infiltration, and better prognosis in the low-risk group.

CONCLUSION

This study showed that was overexpressed in LUAD and was significantly associated with poor prognosis, indicating that may be used as a potential novel biomarker for the diagnosis and prognosis of LUAD. is associated with immune infiltration of LUAD and it is reasonable to speculate that it plays an important role in tumor proliferation and spread. In view of the significant differences in expression between different biological activities, LUAD patients with overexpression may obtain more precise treatment strategies in the clinic.