Faculty of New Sciences & Technologies, Semnan University, Semnan, Iran.
School of Chemical Engineering, College of Engineering, University of Tehran, P.O. Box 11155/4563, Tehran, Iran.
Future Med Chem. 2022 Nov;14(21):1561-1581. doi: 10.4155/fmc-2022-0027. Epub 2022 Oct 27.
Advancements in nanotechnology have resulted in the introduction of several nonviral delivery vectors for the nontoxic, efficient delivery of encapsulated mRNA-based vaccines. Lipid- and polymer-based nanoparticles (NP) have proven to be the most potent delivery systems, providing increased delivery efficiency and protection of mRNA molecules from degradation. Here, the authors provide an overview of the recent studies carried out using lipid NPs and their functionalized forms, polymeric and lipid-polymer hybrid nanocarriers utilized mainly for the encapsulation of mRNAs for gene and immune therapeutic applications. A microfluidic system as a prevalent methodology for the preparation of NPs with continuous flow enables NP size tuning, rapid mixing and production reproducibility. Continuous-flow microfluidic devices for lipid and polymeric encapsulated RNA NP production are specifically reviewed.
纳米技术的进步促使出现了多种非病毒传递载体，用于实现无毒、高效传递包裹的基于 mRNA 的疫苗。脂质和聚合物纳米颗粒 (NP) 已被证明是最有效的传递系统，可提高传递效率并保护 mRNA 分子免受降解。作者在这里概述了最近使用脂质 NP 及其功能化形式、主要用于包裹基因和免疫治疗应用 mRNA 的聚合物和脂质-聚合物杂化纳米载体进行的研究。微流控系统作为一种流行的方法，可用于制备具有连续流的 NPs，实现 NP 尺寸调整、快速混合和生产重现性。具体综述了用于脂质和聚合物包裹 RNA NP 生产的连续流微流控装置。
