[Chlamydosporulation in "Candida albicans": influence of sowing density and glucose, nitrogen, biotin and mineral salts concentration in rice-cream decoction (author's transl)].

Chlamydospore production by Candida albicans was investigated in rice cream decoction inoculated with a blastospore suspension. Effects of glucose, KH2PO4, (NH4)2SO4 and biotin addition were estimated using a "chlamydospore index" i.e. the mean-number of mature chlamydospores produced from a single blastospore development. If no other component was added, it appeared that better sporulation was obtained with both glucose concentration and inoculum size optimum (0.5 g/l and 7.8 X 10(3) blastospores/ml respectively). In glucose-KH2PO4 enriched medium, chlamydosporulation was the best with 0.5 g/l glucose and 0.5 g/l KH2PO4. The addition of biotin reduced the chlamydospore index at the concentrations tested (0.6 to 20 microgram/l). When glucose and (NH4)2SO4 were added together, it seemed that one of the C or N component had to be in low concentration for a good sporulation. The action of salts was also investigated; and finally a synthetic medium is proposed. The improvement of this medium is now in progress.