Department of Biomedical Engineering, University of California, Irvine, Irvine, California, United States of America.
Department of Mathematics, and Department of Physics and Astronomy, University of California, Irvine, Irvine, California, United States of America.
PLoS One. 2022 Oct 27;17(10):e0275804. doi: 10.1371/journal.pone.0275804. eCollection 2022.
Time-lapse microscopy plays critical roles in the studies of cellular dynamics. However, setting up a time-lapse movie experiments is not only laborious but also with low output, mainly due to the cell-losing problem (i.e., cells moving out of limited field of view), especially in a long-time recording. To overcome this issue, we have designed a cost-efficient way that enables cell patterning on the imaging surfaces without any physical boundaries. Using mouse embryonic stem cells as an example system, we have demonstrated that our boundary-free patterned surface solves the cell-losing problem without disturbing their cellular phenotype. Statistically, the presented system increases the effective-throughput of time-lapse microscopy experiments by an order of magnitude.
延时显微镜在细胞动力学研究中起着至关重要的作用。然而，设置延时电影实验不仅繁琐，而且产量低，主要是因为细胞丢失问题（即细胞移出有限的视野），尤其是在长时间记录时。为了解决这个问题，我们设计了一种经济高效的方法，可以在没有任何物理边界的成像表面上对细胞进行图案化。以小鼠胚胎干细胞为例，我们证明了我们的无边界图案化表面可以解决细胞丢失问题，而不会干扰其细胞表型。从统计学上看，该系统将延时显微镜实验的有效通量提高了一个数量级。
