Laboratory of Aquatic Pathobiology, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark.
PLoS One. 2022 Oct 27;17(10):e0276895. doi: 10.1371/journal.pone.0276895. eCollection 2022.
Marker-assisted selective breeding of fish with higher levels of resistance towards specific pathogens may improve fish health, but the impact of host genotype on susceptibility to multiple pathogen infections is still poorly investigated. This study examined the resistance in rainbow trout Oncorhynchus mykiss towards infection with the eye fluke Diplostomum pseudospathaceum. We used genetically selected rainbow trout, carrying SNPs associated with resistance towards the parasitic ciliate Ichthyophthirius multifiliis, and exposed the fish to eye fluke cercariae. We showed that fish partly resistant to I. multifiliis were more susceptible to eye fluke invasion. The expression of immune relevant genes (encoding innate and adaptive factors) was also affected as these genotypes responded less strongly to a secondary fluke infection. The complexity of genome architecture in disease resistance towards multiple pathogens is discussed.
利用标记辅助选择具有更高抗性的鱼类针对特定病原体可能会改善鱼类健康，但宿主基因型对多种病原体感染易感性的影响仍未得到充分研究。本研究检查了虹鳟鱼 Oncorhynchus mykiss 对眼吸虫 Diplostomum pseudospathaceum 感染的抗性。我们使用遗传选择的虹鳟鱼，携带与抗寄生虫纤毛虫 Ichthyophthirius multifiliis 相关的 SNP，并将鱼暴露于眼吸虫尾蚴。我们表明，对 Ichthyophthirius multifiliis 部分有抗性的鱼更容易受到眼吸虫的侵袭。免疫相关基因（编码先天和适应性因素）的表达也受到影响，因为这些基因型对二次吸虫感染的反应较弱。还讨论了针对多种病原体的疾病抗性中基因组结构的复杂性。
