CiLiQuant：基于环状或线性转录本来源对RNA连接 reads 进行定量分析
CiLiQuant: Quantification of RNA Junction Reads Based on Their Circular or Linear Transcript Origin.
作者信息
Morlion Annelien, Hulstaert Eva, Vromman Marieke, Anckaert Jasper, Everaert Celine, Vandesompele Jo, Mestdagh Pieter
机构信息
Department of Biomolecular Medicine, Ghent University, Ghent, Belgium.
OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent, Belgium.
出版信息
Front Bioinform. 2022 Feb 22;2:834034. doi: 10.3389/fbinf.2022.834034. eCollection 2022.
Abstract
Distinguishing circular RNA reads from reads derived from the linear host transcript is a challenging task because of sequence overlap. We developed a computational approach, CiLiQuant, that determines the relative circular and linear abundance of transcripts and gene loci using back-splice and unambiguous forward-splice junction reads generated by existing mapping and circular RNA discovery tools.
摘要
由于序列重叠，区分环状RNA reads与来自线性宿主转录本的reads是一项具有挑战性的任务。我们开发了一种计算方法CiLiQuant，它使用现有映射和环状RNA发现工具生成的反向剪接和明确的正向剪接连接reads来确定转录本和基因座的相对环状和线性丰度。
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