纳米比亚一家屠宰场牛组织中的血清阳性率及分子检测
Seroprevalence, and molecular detection of in cattle tissues from an abattoir in Namibia.
作者信息
Madzingira Oscar, Aikukutu Gottlieb, Kandongo Fidelis, Kolo Francis B, Khaiseb Siegfried, Tjipura-Zaire Georgina, Kabajani Juliet N, Musilika-Shilongo Albertina, van Heerden Henriette
机构信息
Department of Paraclinical Sciences, School of Veterinary Medicine, Faculty of Health Sciences and Veterinary Medicine, University of Namibia, Windhoek, Namibia.
Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
出版信息
Infect Ecol Epidemiol. 2023 Jun 28;13(1):2229589. doi: 10.1080/20008686.2023.2229589. eCollection 2023.
Brucellosis is a worldwide zoonosis that is endemic in Namibia. This study estimated seroprevalence of brucellosis, and determined the presence of infection in slaughtered cattle using the genus-specific 16-23S rRNA interspacer PCR (ITS-PCR), and the species-specific AMOS-PCR. Between December 2018 and May 2019, sera ( 304), pooled lymph nodes ( 304), and individual spleen ( 304) were collected from slaughtered cattle from 52 farms. Sera were tested for anti-Brucella antibodies using the Rose Bengal test (RBT), and the complement fixation test (CFT). Seroprevalence was 2.3% (7/304) (RBT) and 1.6% (5/304) (CFT). Prevalence of positive herds was 9.6% (5/52). Lymph node ( 200) and spleen ( 200) samples from seronegative cattle tested negative for spp. DNA on ITS-PCR, but spp. DNA was detected in lymph nodes (85.7%, 6/7) and spleen (85.7%, 6/7) from RBT positive cattle. ITS-PCR confirmed isolates from lymph node (51.4%, 4/7) and spleen (85.7%, 6/7) as spp.; while AMOS-PCR and species specific (BaSS) PCR confirmed the isolates as , and field strains, respectively. Provision of adequate protective gear, and the promotion of brucellosis awareness among abattoir workers is recommended to prevent zoonotic infection.
布鲁氏菌病是一种在全球范围内流行的人畜共患病，在纳米比亚呈地方性流行。本研究估计了布鲁氏菌病的血清阳性率，并使用属特异性16 - 23S rRNA间隔区PCR（ITS - PCR）和种特异性AMOS - PCR检测屠宰牛中感染的存在情况。在2018年12月至2019年5月期间，从52个农场的屠宰牛中采集了血清（304份）、汇集淋巴结（304份）和单个脾脏（304份）。使用虎红平板凝集试验（RBT）和补体结合试验（CFT）检测血清中的抗布鲁氏菌抗体。血清阳性率为2.3%（7/304）（RBT）和1.6%（5/304）（CFT）。阳性畜群的患病率为9.6%（5/52）。血清学阴性牛的淋巴结（200份）和脾脏（200份）样本在ITS - PCR检测中布鲁氏菌属DNA呈阴性，但在RBT阳性牛的淋巴结（85.7%，6/7）和脾脏（85.7%，6/七）中检测到布鲁氏菌属DNA。ITS - PCR确认来自淋巴结（51.4%，4/7）和脾脏（85.7%，6/7）的分离株为布鲁氏菌属；而AMOS - PCR和布鲁氏菌物种特异性（BaSS）PCR分别确认分离株为流产布鲁氏菌和田间菌株。建议提供足够的防护装备，并提高屠宰场工人对布鲁氏菌病的认识，以预防人畜共患感染。
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