Neill S D, Reid R L, Weatherup S T, Ellis W A
Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Apr;264(1-2):137-44. doi: 10.1016/s0176-6724(87)80133-5.
Thirty reference Leptospira strains and twelve leptospire-like cultures were examined for aminopeptidase activity using twenty-two aminoacyl-beta-naphthylamide substrates. Aminopeptidase activity was demonstrated in extracts of each of the cultures and their substrate specificity profiles compared using several computer analysis procedures. The specificity profiles were consistent for each of the strains examined. Leptospira were readily differentiated from non Leptospira strains. This was supported by DNA base ratios calculated for the strains. Of the non Leptospira strains, seven appeared similar to the "L. illini" cultured included. Two of these strains, which included the strain "L. parva" sp. nov., formed a subgroup of this "L. illini" group. Substrate specificity profiles for the L. interrogans and L. biflexa strains examined were also different from each other.
使用二十二种氨酰基-β-萘酰胺底物，对30株参考钩端螺旋体菌株和12种类钩端螺旋体培养物进行了氨肽酶活性检测。在每种培养物的提取物中均显示出氨肽酶活性，并使用几种计算机分析程序比较了它们的底物特异性谱。所检测的每种菌株的特异性谱都是一致的。钩端螺旋体很容易与非钩端螺旋体菌株区分开来。这得到了为这些菌株计算的DNA碱基比率的支持。在非钩端螺旋体菌株中，有7株与所包括的“伊利诺伊钩端螺旋体”培养物相似。其中两株，包括“细小钩端螺旋体”新种，形成了这个“伊利诺伊钩端螺旋体”组的一个亚组。所检测的问号钩端螺旋体和双曲钩端螺旋体菌株的底物特异性谱也彼此不同。
