Yang Man, Zhang Run, Liu Xiance, Shi Guohua, Liu Hai, Wang Ligang, Hou Xinhua, Shi Lijun, Wang Lixian, Zhang Longchao
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
Beijing Heiliu Animal Husbandry Technology Co, Ltd, Beijing, China.
Anim Genet. 2023 Feb;54(1):24-34. doi: 10.1111/age.13270. Epub 2022 Oct 28.
Increasing intramuscular fat (IMF) content can enhance the sensory quality of meat, including tenderness, juiciness, flavor, and color. Genome-wide association study and RNA-sequencing (RNA-seq) analysis were used to identify candidate IMF genes in Beijing Black pigs, a popular species among consumers in northern China. Two and three single nucleotide polymorphisms were significantly associated with IMF in SSC13 and SSC15 respectively. Solute carrier family 4 member 7 (SLC4A7) on SSC13 and insulin induced gene 2 (INSIG2), coiled-coil domain containing 93 (CCDC93), and diazepam binding inhibitor acyl-CoA binding protein (DBI) on SSC15 are good candidate genes in this population. Furthermore, RNA-seq analysis was performed between high and low IMF groups, and 534 differentially expressed genes were identified. In addition, based on differentially expressed genes, Kyoto Encyclopedia of Genes and Genomes analysis revealed that peroxisome proliferator-activated receptors and FoxO signaling pathway pathways might contribute to IMF. Moreover, the DBI gene was identified as a candidate for IMF both by genome-wide association study and RNA-seq analysis, suggesting that it might be a crucial candidate gene for influencing IMF in Beijing Black pigs.
增加肌内脂肪（IMF）含量可提高肉的感官品质，包括嫩度、多汁性、风味和色泽。全基因组关联研究和RNA测序（RNA-seq）分析被用于鉴定北京黑猪中的IMF候选基因，北京黑猪是中国北方消费者喜爱的品种。分别在13号和15号染色体上有2个和3个单核苷酸多态性与IMF显著相关。13号染色体上的溶质载体家族4成员7（SLC4A7）以及15号染色体上的胰岛素诱导基因2（INSIG2）、含卷曲螺旋结构域93（CCDC93）和地西泮结合抑制剂酰基辅酶A结合蛋白（DBI）是该群体中的良好候选基因。此外，对高IMF组和低IMF组进行了RNA-seq分析，鉴定出534个差异表达基因。另外，基于差异表达基因，京都基因与基因组百科全书分析表明，过氧化物酶体增殖物激活受体和FoxO信号通路可能与IMF有关。此外，DBI基因通过全基因组关联研究和RNA-seq分析均被鉴定为IMF的候选基因，这表明它可能是影响北京黑猪IMF的关键候选基因。
