Hou Renda, Chen Li, Liu Xiance, Liu Hai, Shi Guohua, Hou Xinhua, Zhang Run, Yang Man, Niu Naiqi, Wang Lixian, Zhang Longchao
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Chongqing Academy of Animal Science, Chongqing 402460, China.
Biology (Basel). 2022 Nov 8;11(11):1635. doi: 10.3390/biology11111635.
Loin muscle area (LMA) is an important meat production trait and plays a key role in determining carcass leanness. Genome-wide association study (GWAS) and RNA sequencing (RNA-seq) analysis were used to identify candidate LMA genes in Beijing Black pigs, a popular breed among consumers in northern China. Ten single nucleotide polymorphisms (SNPs) in (SSC) 9 were significantly associated with LMA. These SNPs were mapped to a 2.90 Mb (84.94-87.84 Mb) region. A total of 11 annotated genes were mapped on this region, namely 2, , 1, 72, 2, 2, 13, 2, , 13, and 9. In addition, RNA-seq analysis was performed between the high- and low-LMA groups, and 329 differentially expressed genes (DEGs) were identified. Further, Kyoto Encyclopedia of Genes and Genomes analysis based on DEGs revealed that the JAK/STAT signaling pathway and oxytocin signaling pathway may be responsible for LMA. Both GWAS and RNA-seq analysis identified the 9 gene, indicating that it may be an important candidate gene affecting LMA in Beijing Black pigs. The findings provide valuable molecular insights into the mechanisms that influence LMA content in pigs, which can be utilized in targeted approaches to enhance meat quality and commercial profitability.
腰大肌面积（LMA）是一个重要的产肉性状，在决定胴体瘦肉率方面起着关键作用。采用全基因组关联研究（GWAS）和RNA测序（RNA-seq）分析来鉴定北京黑猪（中国北方消费者喜爱的品种）中影响LMA的候选基因。9号染色体上的10个单核苷酸多态性（SNP）与LMA显著相关。这些SNP定位到一个2.90 Mb（84.94 - 87.84 Mb）的区域。该区域共定位了11个注释基因，分别为2、、1、72、2、2、13、2、、13和9。此外，对高LMA组和低LMA组进行了RNA-seq分析，鉴定出329个差异表达基因（DEG）。进一步基于DEG的京都基因与基因组百科全书分析表明，JAK/STAT信号通路和催产素信号通路可能与LMA有关。GWAS和RNA-seq分析均鉴定出9号基因，表明它可能是影响北京黑猪LMA的一个重要候选基因。这些发现为影响猪LMA含量的机制提供了有价值的分子见解，可用于针对性地提高肉质和商业收益。
