Botany Department, Faculty of Science, Aswan University, Aswan, 81528, Egypt.
World J Microbiol Biotechnol. 2022 Oct 28;38(12):248. doi: 10.1007/s11274-022-03441-y.
Drought severity and duration are expected to increase as a result of ongoing global climate change. Therefore, finding solutions to help plants to deal with drought stress and to improve growth in the face of limited water resources is critical. In this study, a drought tolerant- plant growth promoting endophytic bacterium was isolated from Aloe vera roots. It was identified as Sphingobacterium changzhouense based on 16S rRNA gene sequencing and was deposited into NCBI database with accession number (ON944028). The effect of S. changzhouense inoculation on maize growth under drought stress was investigated. The results revealed that inoculation significantly (p ≤ 0.05) enhanced root and shoot elongation by 205 and 176.19% respectively. Photosynthesis rate, stomatal conductance and water use efficiency were improved in inoculated plants. interestingly, inoculation resulted in significant increase in total chlorophyll, total carbohydrates, proline, total proteins, total phenolics and total flavonoids by 64, 31.5, 25.1, 75.07, 83.7 and 65.4% respectively. Total antioxidant capacity of inoculated plants (51.2 mg/g FW) was higher than that of non-inoculated plants (11.87 mg/g FW), which was found to be positively correlated to the levels of phenolics and flavonoids. Our finding suggests that S. changzhouense could be used to improve crop growth and assist plants to resist drought stress in arid agricultural lands.
由于全球气候变化的持续影响，预计干旱的严重程度和持续时间将会增加。因此，寻找解决方案帮助植物应对干旱胁迫并在有限的水资源条件下提高生长是至关重要的。本研究从库拉索芦荟根部分离出一种具有耐旱和促进植物生长特性的内生细菌，根据 16S rRNA 基因测序将其鉴定为中华动胶菌，并将其保藏于 NCBI 数据库，登录号为 ON944028。研究了中华动胶菌接种对玉米在干旱胁迫下生长的影响。结果表明，接种显著（p≤0.05）促进了根和芽的伸长，分别提高了 205%和 176.19%。接种植物的光合作用速率、气孔导度和水分利用效率均得到提高。有趣的是，接种导致总叶绿素、总碳水化合物、脯氨酸、总蛋白质、总酚和总类黄酮分别增加了 64%、31.5%、25.1%、75.07%、83.7%和 65.4%。接种植物的总抗氧化能力（51.2 mg/g FW）高于未接种植物（11.87 mg/g FW），与酚类和类黄酮的水平呈正相关。我们的研究结果表明，中华动胶菌可用于提高作物生长，并帮助植物在干旱的农业土地中抵御干旱胁迫。
