Chemistry Department, Faculty of Science, Helwan University, Ain-Helwan, Cairo 11795, Egypt.
Photochemistry Department, Chemical Industries Research Institute, National Research Centre, Scopus affiliation ID 60014618, 33 EL Buhouth St., Dokki, Giza 12622, Egypt.
Int J Biol Macromol. 2023 Jan 1;224:858-870. doi: 10.1016/j.ijbiomac.2022.10.172. Epub 2022 Oct 25.
Polymer dots (PDs) ingrained from biopolymers are characterized by their biocompatibility & non-toxicity to be superiorly applicable for biomedicines. The point of novelty in the current study is to focus on the effect of Maillard reaction for conjugation of chitosan with glucose to enhance the affinity of chitosan as a biological resource of PDs. Chitosan-glucose conjugate was firstly prepared by Maillard reaction. PDs were nucleated from chitosan (C1 acidic, 10.6 nm & C2 basic, 11.4 nm) and chitosan-glucose conjugate (C3 acidic, 6.8 nm & C4 basic, 5.7 nm). The affinity of chitosan versus chitosan-glucose conjugate as molecular precursors for PDs as antiviral and anticancer laborers was studied. The synthesized PDs were tested against lung cancer (NSCLC, A549) and the estimated IC was 282.4 & 165.4 μg/mL for PDs (C3 & C4) ingrained from chitosan-glucose conjugate. The antiviral action of PDs against Coronavirus (229E) was estimated and the obtained IC for C3 & C4 was 43.6 and 19.3 mg/mL, respectively. PDs ingrained from chitosan-glucose conjugate showed higher anticancer and antiviral activities compared to that clustered from chitosan. Consequently, the modification of chitosan via Maillard reaction enhanced the biological affinity of the obtained PDs to be effectively applicable as antitumor and antiviral laborers.
聚合物点（PDs）由生物聚合物衍生而来，具有生物相容性和低毒性，非常适用于生物医学。本研究的新颖之处在于关注美拉德反应对壳聚糖与葡萄糖的结合，以增强壳聚糖作为 PDs 生物资源的亲和力。首先通过美拉德反应制备壳聚糖-葡萄糖缀合物。从壳聚糖（C1 酸性，10.6nm 和 C2 碱性，11.4nm）和壳聚糖-葡萄糖缀合物（C3 酸性，6.8nm 和 C4 碱性，5.7nm）中引发 PDs。研究了壳聚糖与壳聚糖-葡萄糖缀合物作为 PDs 的分子前体在抗病毒和抗癌方面的亲和力。合成的 PDs 对肺癌（NSCLC，A549）进行了测试，PDs（C3 和 C4）从壳聚糖-葡萄糖缀合物中沉淀的 IC 值分别为 282.4 和 165.4μg/mL。对 PDs 针对冠状病毒（229E）的抗病毒作用进行了评估，C3 和 C4 的 IC 值分别为 43.6 和 19.3mg/mL。与从壳聚糖聚集的 PDs 相比，从壳聚糖-葡萄糖缀合物中沉淀的 PDs 表现出更高的抗癌和抗病毒活性。因此，通过美拉德反应修饰壳聚糖增强了所得 PDs 的生物亲和力，可有效用作抗肿瘤和抗病毒剂。
