Physics Department, Faculty of Science, Damanhour University, Damanhour, El-Beheira, Egypt.
Botany Department, Faculty of Science, Tanta University, Tanta, 31527, Egypt.
BMC Microbiol. 2023 Sep 29;23(1):274. doi: 10.1186/s12866-023-03018-1.
Microbial skin infections, antibiotic resistance, and poor wound healing are major problems, and new treatments are needed. Our study targeted solving this problem with Nigella sativa (NS) oil and photodynamic therapy based on methylene blue (MB-PDT). Antibacterial activity and minimum inhibitory concentration (MIC) were determined via agar well diffusion assay and broth microdilution, respectively. Transmission electron microscopy (TEM) proved deformations in Staphylococcus aureus ATCC 6538. Gas chromatography-mass spectrometry identified useful compounds that were suggested to be responsible for the potency of the oil. NS oil was tested as an antivirus against low pathogenic coronavirus (229E). Therapies examined, MB-PDT, NS, and MB-PDT + NS oil, to accelerate wound healing. The antibacterial efficacy against S. aureus was promising, with a MIC of 12.5% and TEM showing injured cells treated with NS oil. This oil inhibited 229E virus up to 42.85% and 32.14%. All tested therapies were successful in accelerating wound healing. The most successful was combined therapy (MB-PDT + NS oil), with a faster healing time. The combined therapy (MB-PDT + NS oil) reduced bacterial counts, which may be a key factor in accelerating wound healing. Skin wound histology was investigated; blood hematology and biochemical analysis did not change significantly after the safe combination treatment. A combination treatment could facilitate healing in a simple and inexpensive way in the future. Based on the results of the in vitro and in vivo studies, it was determined that NS oil had antibacterial and anti-corona virus activity when used in conjunction with photodynamic treatment based on methylene blue to treat wound infections.
微生物皮肤感染、抗生素耐药性和伤口愈合不良是主要问题，需要新的治疗方法。我们的研究旨在使用黑种草籽油（NS）和基于亚甲蓝的光动力疗法（MB-PDT）解决这个问题。通过琼脂孔扩散法和肉汤微量稀释法分别测定了抗菌活性和最小抑菌浓度（MIC）。透射电子显微镜（TEM）证明了金黄色葡萄球菌 ATCC 6538 的变形。气相色谱-质谱联用仪鉴定了有用的化合物，这些化合物被认为是油的功效的原因。NS 油被测试作为一种抗病毒药物，用于低致病性冠状病毒（229E）。检查的治疗方法包括 MB-PDT、NS 和 MB-PDT+NS 油，以加速伤口愈合。对金黄色葡萄球菌的抗菌效果很有前景，MIC 为 12.5%，TEM 显示 NS 油处理后的受损细胞。这种油抑制 229E 病毒高达 42.85%和 32.14%。所有测试的治疗方法都成功地加速了伤口愈合。最成功的是联合治疗（MB-PDT+NS 油），愈合时间更快。联合治疗（MB-PDT+NS 油）减少了细菌数量，这可能是加速伤口愈合的关键因素。对皮肤伤口组织学进行了研究；在安全联合治疗后，血液血液学和生化分析没有显著变化。联合治疗可能会以简单且廉价的方式促进未来的愈合。基于体外和体内研究的结果，确定 NS 油在与基于亚甲蓝的光动力疗法联合使用时具有抗菌和抗冠状病毒活性，用于治疗伤口感染。
