Høverstad T, Midtvedt T
Acta Pathol Microbiol Immunol Scand B. 1987 Jun;95(3):211-2. doi: 10.1111/j.1699-0463.1987.tb03114.x.
Short-chain fatty acids (SCFAs) were analysed by gas chromatography in 7 sterile fluid thioglycollate media. The total SCFA concentration varied from 250 mumol/l to 1720 mumol/l. Acetic acid (52.0%-91.3% of total SCFA concentration) and n-butyric acid (5.8%-48.0% of total concentration) were the main acids in all sterile media; low concentrations of propionic, isobutyric, isovaleric, and n-valeric acid were present in most media. In studies of bacterial metabolism, or when analyses of SCFAs are used for identification of bacteria, testing of the sterile medium is necessary.
通过气相色谱法对7种无菌液体硫乙醇酸盐培养基中的短链脂肪酸(SCFAs)进行了分析。总SCFA浓度在250μmol/L至1720μmol/L之间变化。乙酸(占总SCFA浓度的52.0%-91.3%)和正丁酸(占总浓度的5.8%-48.0%)是所有无菌培养基中的主要酸类;大多数培养基中存在低浓度的丙酸、异丁酸、异戊酸和正戊酸。在细菌代谢研究中,或者当使用SCFAs分析来鉴定细菌时,对无菌培养基进行检测是必要的。