Liao Z, Lai D
Department of Microbiology, Guangdong Pharm. College, Guangzhou, 510224.
Se Pu. 1997 May;15(3):216-8.
With chromatograph produced in our country, the gas chromatograms of fatty acid metabolites of five strains of Clostridium difficile isolated from stool of patients with tumor and one strain for control were compared and analysed by using stainless steel chromatographic column, 15% modified polyethylene glycol stationary phase nitrogen carriergas 140 degrees C column temperature and 190 degrees C detector temperature. The chromatograms of the isolated strains and the control strain were found to be extremely similar. All of them were peaks of acetic, propionic, isobutyric, butyric, isovaleric, valeric, and isocaproic acid. In comparison, the peaks of acetic, butyric, and isocaproic acid were higher, those of the isobutyric and isovaleric acid were medium and those of propionic and valeric acid were lower. Moreover, a low, flat peak appeared at 14th minute and was similar to that of p-cresol reported by Berg et al. The results of our experiment revealed that gas chromatography may be used as a simple, quick method for the identification of Clostridium difficile.
采用国产色谱仪，使用不锈钢色谱柱、15%改性聚乙二醇固定相、氮气载气、柱温140℃、检测温度190℃，对从肿瘤患者粪便中分离出的5株艰难梭菌及1株对照菌的脂肪酸代谢产物的气相色谱图进行了比较和分析。结果发现，分离菌株和对照菌株的色谱图极为相似。它们均为乙酸、丙酸、异丁酸、丁酸、异戊酸、戊酸和异己酸的峰。相比之下，乙酸、丁酸和异己酸的峰较高，异丁酸和异戊酸的峰中等，丙酸和戊酸的峰较低。此外，在第14分钟出现了一个低而平的峰，与Berg等人报道的对甲酚的峰相似。我们的实验结果表明，气相色谱法可作为一种简单、快速的艰难梭菌鉴定方法。
