Shanghai Engineering Research Center for Food Rapid Detection, School of Health Science and Engineering, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai, 200093, People's Republic of China.
Appl Microbiol Biotechnol. 2022 Dec;106(23):7819-7829. doi: 10.1007/s00253-022-12249-7. Epub 2022 Oct 29.
Komagataella phaffii (syn. Pichia pastoris), a methylotrophic yeast, has many advantages as a protein expression system, but has the disadvantage of hazardous methanol as an inducer and carbon source. To enable substitution of formate for methanol, a formate assimilation pathway was constructed by the co-expression of acetyl-CoA synthase, acetaldehyde dehydrogenase, and transcription factor Mit1, resulting in a 103.5 ± 12.5% increase in xylanase production. Recombinant K. phaffii was able to use formate as a carbon source, indicating successful substitution of formate for methanol. Xylanase production, using the safe and sustainable formate as an inducer and carbon source, is a major advance in the field of industrial enzyme production. KEY POINTS: • Change to formate assimilation by recombinant K. phaffii instead of methanol • K. phaffii expressed xylanase by formate induction instead of methanol induction • Increased xylanase expression by transcription factor co-expression.
毕赤酵母（Komagataella phaffii）（同义名：巴斯德毕赤酵母 Pichia pastoris）是一种甲醇营养型酵母，作为蛋白表达系统具有许多优点，但作为诱导剂和碳源的甲醇具有危害性。为了用甲酸盐替代甲醇，通过共表达乙酰辅酶 A 合酶、乙醛脱氢酶和转录因子 Mit1 构建了甲酸盐同化途径，导致木聚糖酶产量增加 103.5±12.5%。重组毕赤酵母能够利用甲酸盐作为碳源，表明甲酸盐成功替代了甲醇。使用安全可持续的甲酸盐作为诱导剂和碳源进行木聚糖酶生产，是工业酶生产领域的重大进展。关键点：
通过重组毕赤酵母的甲酸盐同化取代甲醇
毕赤酵母通过甲酸盐诱导而非甲醇诱导表达木聚糖酶
通过转录因子共表达增加木聚糖酶表达。
