Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China; The Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China.
J Hazard Mater. 2023 Feb 5;443(Pt A):130233. doi: 10.1016/j.jhazmat.2022.130233. Epub 2022 Oct 21.
Ochratoxin A (OTA), which has strong hepatotoxicity and nephrotoxicity, can accumulate in the human body through the food chain; thus, the selective and effective detection of OTA is urgently required for food security. Nanozymes with hyperfine size and shape control have attracted attention because of their controllable structure and intrinsic activity. Herein, CuFe-bimetal coordinated N-doped carbon (Cu@Fe-NC) with morphology-driven peroxidase-mimicking activity was synthesized using CuO with specific polygonal cubes and fully exposed {111} crystalline facets as the template to produce a CuFe-bimetallic metal organic framework (MOF) and further treating CuFe-MOF with high-temperature pyrolysis. N-doping can confer electronegativity to exhibit high affinity, while the large surface area of the porous carbon support can facilitate rapid adsorption-desorption equilibrium. Using the peroxidase-mimicking Cu@Fe-NC as a carrier, a versatile immunoassay for the detection of OTA was implemented based on the ratiometric fluorescence and the localized surface plasmon resonance peak shift, achieving a detection limit of 0.52 ng/L in the range of 0.001-10 μg/L. Therefore, the strategy of enhancing enzyme-mimicking activity using specific shapes and crystalline facets may open new avenues for food and environmental analysis.
赭曲霉毒素 A（OTA）具有很强的肝毒性和肾毒性，可通过食物链在人体内积累；因此，迫切需要对食品的 OTA 进行选择性和有效的检测。由于具有可控的结构和内在活性，具有超精细尺寸和形状控制的纳米酶引起了人们的关注。在此，使用具有特定多边形立方体形貌和完全暴露的 {111} 晶面的 CuO 作为模板，合成了形态驱动的过氧化物酶模拟活性的 CuFe 双金属配位 N 掺杂碳（Cu@Fe-NC），以生成 CuFe 金属有机骨架（MOF），然后用高温热解进一步处理 CuFe-MOF。N 掺杂可以赋予电负性，表现出高亲和力，而多孔碳载体的大表面积可以促进快速吸附-解吸平衡。通过使用过氧化物酶模拟的 Cu@Fe-NC 作为载体，实现了基于比率荧光和局域表面等离子体共振峰位移的 OTA 的多功能免疫测定，在 0.001-10μg/L 的范围内检测限为 0.52ng/L。因此，使用特定形状和晶面来增强酶模拟活性的策略可能为食品和环境分析开辟新途径。
