IGEPP-UMR 1349, INRAE, Institut Agro, Univ Rennes 1, Rennes, France.
Innolea, 6 Chemin de Panedautes, Mondonville, France.
Pest Manag Sci. 2024 May;80(5):2235-2240. doi: 10.1002/ps.7266. Epub 2022 Nov 11.
Improving crop resistance to insect herbivores is a major research objective in breeding programs. Although genomic technologies have increased the speed at which large populations can be genotyped, breeding programs still suffer from phenotyping constraints. The pollen beetle (Brassicogethes aeneus) is a major pest of oilseed rape for which no resistant cultivar is available to date, but previous studies have highlighted the potential of white mustard as a source of resistance and introgression of this resistance appears to be a promising strategy. Here we present a phenotyping protocol allowing mid-throughput (i.e., increased throughput compared to current methods) acquisition of resistance data, which could then be used for genetic mapping of QTLs.
Contrasted white mustard genotypes were selected from an initial field screening and then evaluated for their resistance under controlled conditions using a standard phenotyping method on entire plants. We then upgraded this protocol for mid-throughput phenotyping, by testing two alternative methods. We found that phenotyping on detached buds did not provide the same resistance contrasts as observed with the standard protocol, in contrast to the phenotyping protocol with miniaturized plants. This protocol was then tested on a large panel composed of hundreds of plants. A significant variation in resistance among genotypes was observed, which validates the large-scale application of this new phenotyping protocol.
The combination of this mid-throughput phenotyping protocol and white mustard as a source of resistance against the pollen beetle offers a promising avenue for breeding programs aiming to improve oilseed rape resistance. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
提高作物对昆虫食草动物的抗性是育种计划的主要研究目标。尽管基因组技术提高了对大量种群进行基因分型的速度，但育种计划仍然受到表型分析的限制。花粉甲虫（Brassicogethes aeneus）是一种重要的油菜害虫，目前还没有可用的抗虫品种，但之前的研究已经强调了白芥菜作为抗性来源的潜力，并且这种抗性的基因渗入似乎是一种很有前途的策略。在这里，我们提出了一种表型分析方案，允许进行高通量（即与当前方法相比，增加了通量）抗性数据的获取，然后可以将这些数据用于 QTL 的遗传作图。
从最初的田间筛选中选择了对比鲜明的白芥菜基因型，然后在受控条件下使用标准表型分析方法对整个植株进行抗性评估。然后，我们通过测试两种替代方法对该方案进行了高通量表型分析的升级。我们发现，与标准方案相比，分离芽的表型分析没有提供相同的抗性对比，而微型化植株的表型分析则不同。然后，该方案在由数百株植物组成的大型面板上进行了测试。观察到基因型之间存在显著的抗性差异，这验证了这种新表型分析方案的大规模应用。
将这种高通量表型分析方案与白芥菜作为抗花粉甲虫的抗性来源相结合，为旨在提高油菜抗性的育种计划提供了一个有前途的途径。
