益生菌对复原乳中黄曲霉毒素M1的解毒作用 ， 以及 。 （感觉原文表述不太完整准确呢，你可以检查下或者补充完整信息让我更精准翻译）
Detoxification of aflatoxin M1 by probiotics , , and in reconstituted milk.
作者信息
Rezasoltani Sama, Amir Ebrahimi Niloufar, Khadivi Boroujeni Reza, Asadzadeh Aghdaei Hamid, Norouzinia Mohsen
机构信息
Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Microbial Biotechnology Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
出版信息
Gastroenterol Hepatol Bed Bench. 2022 Summer;15(3):263-270. doi: 10.22037/ghfbb.v15i3.2402.
AIM
The current study aimed to remove aflatoxin from reconstituted milk by adding three probiotics, namely , , and .
BACKGROUND
Aflatoxins are poisonous substances produced by certain kinds of fungi that are found naturally all over the world. They can contaminate food crops and pose a serious health threat to humans and livestock. Microbial detoxification is one method of eliminating aflatoxins, including aflatoxin M1.
METHODS
For this purpose, about 109 and 107 cfu/ml of casei, and were inoculated into skim milk without aflatoxin M1. The samples were then spiked by aflatoxin M1 in concentrations of 0.5 and 0.75 ng/ml. The concentration of the aflatoxin residing in supernatant of milk samples after different storage times (30 and 90 minutes) and temperatures of 4 ℃ and 37 °C was measured by ELISA method, and the results were confirmed by HPLC.
RESULTS
The results showed that the highest amount of aflatoxin M1 removal was related to (96.88 ± 3.79c) with a microbial density concentration of 109 cfu/ml and toxin concentration of 0.75 ng/ml at 37 °C for 90 minutes and then to (71.46 ± 3.79b) with a microbial density concentration of 107 cfu/ml and toxin concentration 0.75 ng/ml at 4 °C for 90 minutes. Furthermore, the maximum level of AFM1 binding to 107 cfu/ml of with average binding percentages of 64.31 ± 3/79c was 0.75 ng/ml at 37 °C for 90 minutes.
CONCLUSION
The results revealed the possibility of using in combination with the selected probiotics of and in the detoxification of AFM1-contaminated milk.
目的
本研究旨在通过添加三种益生菌，即 、 和 ，去除复原乳中的黄曲霉毒素。
背景
黄曲霉毒素是由某些真菌产生的有毒物质，在世界各地均有天然存在。它们会污染粮食作物，对人类和牲畜构成严重的健康威胁。微生物解毒是消除黄曲霉毒素的一种方法，包括黄曲霉毒素M1。
方法
为此，将约109和107 cfu/ml的干酪乳杆菌、 和 接种到不含黄曲霉毒素M1的脱脂乳中。然后将样品分别添加浓度为0.5和0.75 ng/ml的黄曲霉毒素M1。采用酶联免疫吸附测定法（ELISA）测定不同储存时间（30和90分钟）以及4℃和37℃温度条件下牛奶样品上清液中黄曲霉毒素的浓度，并通过高效液相色谱法（HPLC）进行结果确认。
结果
结果表明，在37℃下90分钟，微生物密度浓度为109 cfu/ml且毒素浓度为0.75 ng/ml时，干酪乳杆菌去除黄曲霉毒素M1的量最高（96.88 ± 3.79c）；其次是在4℃下90分钟，微生物密度浓度为107 cfu/ml且毒素浓度为0.75 ng/ml时的干酪乳杆菌（71.46 ± 3.79b）。此外，在37℃下90分钟，107 cfu/ml的干酪乳杆菌与0.75 ng/ml黄曲霉毒素M1结合的最大水平，平均结合百分比为64.31 ± 3/79c。
结论
结果揭示了将干酪乳杆菌与所选的嗜酸乳杆菌和植物乳杆菌联合用于去除被AFM1污染牛奶中黄曲霉毒素的可能性。
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