Jing Changliang, Wang Jiahao, Xie Yi, Zhang Jianhui, Guo Yixuan, Tian Tian, Tang Jing, Ju Fuzhu, Wang Chunkai, Liu Yanhua, Zhang Zhongfeng, Yang Xingyou, Zhang Hongbo
Key Laboratory of Synthetic Biology of Ministry of Agriculture and Rural Affairs, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China.
Sichuan Tobacco Science Research Institute, Chengdu, China.
Front Microbiol. 2022 Oct 13;13:1026680. doi: 10.3389/fmicb.2022.1026680. eCollection 2022.
Tobacco contains a large amount of bioactive ingredients which can be used as source of feed. The objective of this study was to evaluate the effects of dietary addition of low-nicotine tobacco (LNT) on the growth performance, blood status, cecum microbiota and metabolite composition of meat rabbits. A total of 80 Kangda meat rabbits of similar weight were assigned randomly as four groups, and three of them were supplemented with 5%, 10%, and 20% LNT, respectively, with the other one fed with basal diet as control group. Each experiment group with 20 rabbits was raised in a single cage. The experiments lasted for 40 days with a predictive period of 7 days. The results revealed that LNT supplementation had no significant effect on the growth performance, but increased the half carcass weight compared with control group. Dietary supplemention of LNT decreased the triglycerides and cholesterol content in rabbit serum, and significantly increased the plasma concentration of lymphocytes (LYM), monocytes, eosinophils, hemoglobin HGB and red blood cells. In addition, LNT supplementation significantly changed the microbial diversity and richness, and metagenomic analysis showed that LNT supplementation significantly increased , , and at genus level. Moreover, LC-MS data analysis identified a total of 308 metabolites that markedly differed after LNT addition, with 190 significantly upregulated metabolites and 118 significantly downregulated metabolites. Furthermore, the correlation analysis showed that there was a significant correlation between the microbial difference and the rabbit growth performance. Overall, these findings provide theoretical basis and data support for the application of LNT in rabbits.
烟草含有大量可作为饲料来源的生物活性成分。本研究的目的是评估日粮中添加低尼古丁烟草（LNT）对肉兔生长性能、血液状况、盲肠微生物群和代谢产物组成的影响。将80只体重相近的康大肉兔随机分为四组，其中三组分别添加5%、10%和20%的LNT，另一组饲喂基础日粮作为对照组。每个实验组有20只兔子，单笼饲养。实验持续40天，预试期7天。结果表明，添加LNT对生长性能无显著影响，但与对照组相比，半胴体重增加。日粮中添加LNT可降低兔血清中甘油三酯和胆固醇含量，并显著提高血浆中淋巴细胞（LYM）、单核细胞、嗜酸性粒细胞、血红蛋白HGB和红细胞的浓度。此外，添加LNT显著改变了微生物的多样性和丰富度，宏基因组分析表明，添加LNT在属水平上显著增加了 、 、 和 。此外，LC-MS数据分析共鉴定出308种添加LNT后有显著差异的代谢产物，其中190种显著上调，118种显著下调。此外，相关性分析表明，微生物差异与兔生长性能之间存在显著相关性。总体而言，这些发现为LNT在兔中的应用提供了理论依据和数据支持。
