Luo Qin, Li Na, Xu Jun-Wei
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China.
Faculty of Science, Kunming University of Science and Technology, Kunming, China.
Front Microbiol. 2022 Oct 14;13:1025983. doi: 10.3389/fmicb.2022.1025983. eCollection 2022.
The methyltransferase LaeA is a global regulator involved in the biosynthesis of secondary metabolites by ascomycete fungi. However, little is known of its regulatory role in basidiomycete fungi. In this study, the gene was identified in the basidiomycete and its function in regulating the biosynthesis of anti-tumor ganoderic acids was evaluated. A deletion (ΔlaeA) strain exhibited significantly reduced concentration of ganoderic acids. qRT-PCR analysis further revealed that the transcription levels of genes involved in the biosynthesis of ganoderic acids were drastically lower in the ΔlaeA strain. Moreover, deletion of resulted in decreased accumulation of intermediates and abundances of asexual spores in liquid static culture of . In contrast, constitutive overexpression of resulted in increased concentration of ganoderic acids. These results demonstrate an essential role of LaeA in the regulation of ganoderic acid biosynthesis in .
甲基转移酶LaeA是一种全局调节因子，参与子囊菌次生代谢产物的生物合成。然而，其在担子菌中的调节作用却鲜为人知。在本研究中，在担子菌中鉴定出该基因，并评估了其在调节抗肿瘤灵芝酸生物合成中的功能。一个LaeA缺失（ΔlaeA）菌株显示灵芝酸浓度显著降低。qRT-PCR分析进一步表明，参与灵芝酸生物合成的基因转录水平在ΔlaeA菌株中大幅降低。此外，缺失LaeA导致灵芝在液体静置培养中中间产物积累减少和无性孢子丰度降低。相反，组成型过表达LaeA导致灵芝酸浓度增加。这些结果证明了LaeA在调节灵芝中灵芝酸生物合成中的重要作用。
