Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University , Changchun 130118, China.
Shandong Provincial Key Laboratory of Agricultural Microbiology, College of Plant Protection, Shandong Agricultural University , Tai'an 271018, China.
Mycologia. 2021 Jan-Feb;113(1):12-19. doi: 10.1080/00275514.2020.1810515. Epub 2020 Oct 21.
MADS-box transcription factors play crucial roles in regulating development processes and biosynthesis of secondary metabolites in eukaryotes. However, the role of MADS-box transcription factors vary among fungal species, and their function remains unclear in the medicinally and economically important fungus . In this study, we characterized a MADS-box gene, , in . Analyses using quantitative real-time polymerase chain reaction (qRT-PCR) showed that expression levels were up-regulated from the mycelia to the primordia stage. In order to further evaluate the effect of MADS-box transcription factors on secondary metabolism, we utilized RNA interference (RNAi) to silence in . Ganoderic acid (GA) and flavonoid contents were enhanced in -silenced strains, suggesting that negatively regulates GA and flavonoid accumulation.
MADS 框转录因子在真核生物的发育过程和次生代谢物的生物合成中起着关键作用。然而，MADS 框转录因子在真菌物种中的作用各不相同，其在药用和经济上重要的真菌中的功能尚不清楚。在这项研究中，我们对 中的一个 MADS 框基因进行了表征。使用定量实时聚合酶链反应（qRT-PCR）的分析表明， 的表达水平从菌丝体到原基期上调。为了进一步评估 MADS 框转录因子对次生代谢的影响，我们利用 RNA 干扰（RNAi）沉默 。在沉默菌株中，灵芝酸（GA）和类黄酮含量增加，表明 负调控 GA 和类黄酮的积累。
