Ghafouri-Fard Soudeh, Safarzadeh Arash, Mahmud Hussen Bashdar, Akhavan-Bahabadi Mehdi, Taheri Mohammad, Sharifi Guive
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Pharmacognosy, College of Pharmacy, Hawler Medical University, Erbil, Iraq.
Front Oncol. 2022 Oct 13;12:1024567. doi: 10.3389/fonc.2022.1024567. eCollection 2022.
Glioblastoma multiforme (GBM) is the most frequent malignant type of primary brain cancers and is a malignancy with poor prognosis. Thus, it is necessary to find novel therapeutic modalities based on molecular events occur at different stages of tumor progression. We used expression profiles of GBM tissues that contained long non-coding RNA (lncRNA), microRNA (miRNA) and mRNA signatures to make putative ceRNA networks. Our strategy led to identification of 1080 DEmRNAs, including 777 downregulated DEmRNAs (such as GJB6 and SLC12A5) and 303 upregulated DEmRNAs (such as TOP2A and RRM2), 19 DElncRNAs, including 16 downregulated DElncRNAs (such as MIR7-3HG and MIR124-2HG) and 3 upregulated DElncRNAs (such as CRNDE and XIST) and 49 DEmiRNAs, including 10 downregulated DEmiRNAs (such as hsa-miR-10b-5p and hsa-miR-1290) and 39 upregulated DEmiRNAs (such as hsa-miR-219a-2-3p and hsa-miR-338-5p). We also identified DGCR5, MIAT, hsa-miR-129-5p, XIST, hsa-miR-128-3p, PART1, hsa-miR-10b-5p, LY86-AS1, CRNDE, and DLX6-AS1 as 10 hub genes in the ceRNA network. The current study provides novel insight into molecular events during GBM pathogenesis. The identified molecules can be used as therapeutic targets for GBM.
多形性胶质母细胞瘤（GBM）是原发性脑癌中最常见的恶性类型，且预后不良。因此，有必要基于肿瘤进展不同阶段发生的分子事件寻找新的治疗方法。我们利用包含长链非编码RNA（lncRNA）、微小RNA（miRNA）和信使RNA（mRNA）特征的GBM组织表达谱构建了假定的竞争性内源RNA（ceRNA）网络。我们的策略鉴定出了1080个差异表达mRNA（DEmRNA），包括777个下调的DEmRNA（如GJB6和SLC12A5）和303个上调的DEmRNA（如TOP2A和RRM2），19个差异表达lncRNA（DElncRNA），包括16个下调的DElncRNA（如MIR7-3HG和MIR124-2HG）和3个上调的DElncRNA（如CRNDE和XIST），以及49个差异表达miRNA（DEmiRNA），包括10个下调的DEmiRNA（如hsa-miR-10b-5p和hsa-miR-1290）和39个上调的DEmiRNA（如hsa-miR-219a-2-3p和hsa-miR-338-5p）。我们还在ceRNA网络中鉴定出DGCR5、MIAT、hsa-miR-129-5p,XIST、hsa-miR-128-3p、PART1、hsa-miR-10b-5p、LY86-AS1、CRNDE和DLX6-AS1为10个枢纽基因。本研究为GBM发病机制中的分子事件提供了新的见解。所鉴定的分子可作为GBM的治疗靶点。
