从微小的细胞质活检中进行单细胞时间转录组学研究。

Single-cell temporal transcriptomics from tiny cytoplasmic biopsies.

机构信息

Nanobiosensorics Laboratory, ELKH EK MFA, Budapest, Hungary.

出版信息

Cell Rep Methods. 2022 Oct 17;2(10):100319. doi: 10.1016/j.crmeth.2022.100319. eCollection 2022 Oct 24.

DOI:10.1016/j.crmeth.2022.100319

PMID:36313799

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9606287/

Abstract

In a recent issue in , Chen et al. present Live-seq, a single-cell transcriptomic profiling method using picoliter scale single-cell cytoplasmic biopsies instead of complete cell lysis. Since the cells quickly recover and basically remain unaffected after the cytoplasmic extraction, the authors transform single-cell RNA sequencing (scRNA-seq) from an end point to a temporal analysis platform.

摘要

在最近的一期《》中，Chen 等人提出了 Live-seq 方法，这是一种使用皮升级别的单细胞细胞质活检而不是完整细胞裂解来进行单细胞转录组谱分析的方法。由于细胞质提取后细胞能够迅速恢复，并且基本保持不受影响，作者将单细胞 RNA 测序（scRNA-seq）从终点分析转化为时间分析平台。