Hori M, Nozaki S, Nagami K, Asakura H, Umezawa H
Biochim Biophys Acta. 1978 Nov 21;521(1):101-10. doi: 10.1016/0005-2787(78)90252-6.
Griseolutein acts as a bactericidal agent, its toxicity decreasing with increase in cell density. There is no evidence that griseolutein acts at a specific stage of cell cycle. Inhibition of incorporation of radioactive thymidine into acid-insoluble fraction of cells is marked and rapid, while inhibition of incorporation of uridine also takes place. Incorporation of 32Pi into the acid-insoluble fraction of cells is inhibited while the incorporation into the nucleotide pool is not. Concentration of any of the four deoxyribonucleoside triphosphates in griseolutein-treated cells are similar to or higher than those in untreated cells. No extensive degradation of cellualr DNA is caused by griseolutein. DNA synthesis in plasmolyzed cells in not inhibited by griseolutein.
灰黄蝶呤作为一种杀菌剂，其毒性随细胞密度的增加而降低。没有证据表明灰黄蝶呤作用于细胞周期的特定阶段。放射性胸苷掺入细胞酸不溶性部分的过程受到显著且快速的抑制，同时尿苷的掺入也受到抑制。32Pi掺入细胞酸不溶性部分受到抑制，而掺入核苷酸池则不受影响。经灰黄蝶呤处理的细胞中四种脱氧核糖核苷三磷酸中任何一种的浓度与未处理细胞中的相似或更高。灰黄蝶呤不会导致细胞DNA的广泛降解。质壁分离细胞中的DNA合成不受灰黄蝶呤的抑制。
