Boye E, Alver S, Skarstad K
J Bacteriol. 1981 Mar;145(3):1413-6. doi: 10.1128/jb.145.3.1413-1416.1981.
Escherichia coli cells made permeable with a hypotonic tris(hydroxymethyl)aminomethane buffer utilized exogenous deoxyribonucleoside triphosphates to perform semiconservative replication. The rate of replication was the same as in cells made permeable with toluene or sucrose.
用低渗的三(羟甲基)氨基甲烷缓冲液使大肠杆菌细胞具有通透性,这些细胞利用外源脱氧核糖核苷三磷酸进行半保留复制。复制速率与用甲苯或蔗糖处理后具有通透性的细胞相同。