Division of Vectors and Parasitic Diseases, Korea Disease Control and Prevention Agency, Osong 28159, Korea.
Korean J Parasitol. 2022 Oct;60(5):309-315. doi: 10.3347/kjp.2022.60.5.309. Epub 2022 Oct 21.
Inflammatory bowel disease (IBD) is a chronic and recurrent illness of the gastrointestinal tract. Treatment of IBD traditionally involves the use of aminosalicylic acid and steroids, while these drugs has been associated with untoward effects and refractoriness. The absence of effective treatment regimen against IBD has led to the exploration of new targets. Parasites are promising as an alternative therapy for IBD. Recent studies have highlighted the use of parasite-derived substances, such as excretory secretory products, extracellular vesicles (EVs), and exosomes, for the treatment of IBD. In this report, we examined whether EVs secreted by Giardia lamblia could prevent colitis in a mouse model. G. lamblia EVs (GlEVs) were prepared from in vitro cultures of Giardia trophozoites. Clinical signs, microscopic colon tissue inflammation, and cytokine expression levels were detected to assess the effect of GlEV treatment on dextran sulfate sodium (DSS)-induced experimental murine colitis. The administration of GlEVs prior to DSS challenge reduced the expression levels of pro-inflammatory cytokines, including tumor necrosis factor alpha, interleukin 1 beta, and interferon gamma. Our results indicate that GlEV can exert preventive effects and possess therapeutic properties against DSS-induced colitis.
炎症性肠病（IBD）是一种慢性和复发性胃肠道疾病。IBD 的传统治疗方法包括使用氨基水杨酸和类固醇，而这些药物与不良反应和抗药性有关。由于缺乏有效的 IBD 治疗方案，因此人们一直在探索新的靶点。寄生虫作为 IBD 的替代疗法具有很大的潜力。最近的研究强调了寄生虫衍生物质（如分泌外排产物、细胞外囊泡（EVs）和外泌体）在治疗 IBD 中的应用。在本报告中，我们研究了蓝氏贾第鞭毛虫分泌的 EV 是否可以预防小鼠模型中的结肠炎。从体外培养的蓝氏贾第鞭毛虫滋养体中制备了 G. lamblia EV（GlEVs）。通过检测临床症状、显微镜下结肠组织炎症和细胞因子表达水平，评估 GlEV 治疗对葡聚糖硫酸钠（DSS）诱导的实验性小鼠结肠炎的影响。在 DSS 攻击前给予 GlEV 可降低促炎细胞因子（包括肿瘤坏死因子 alpha、白细胞介素 1 beta 和干扰素 gamma）的表达水平。我们的结果表明，GlEV 可以对 DSS 诱导的结肠炎发挥预防作用并具有治疗作用。
