Seyfi Abad Shapouri Masoud Reza, Mahmoodi Pezhman, Ghorbanpour Najafabadi Masoud, Haji Haji-Kolaei Mohammad Rahim, Moradi Choghakabodi Parastoo, Lotfi Mohsen, Pourmahdi Boroujeni Mahdi, Ekhtelat Maryam, Daghari Maryam
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran.
Vet Res Forum. 2022 Sep;13(3):403-407. doi: 10.30466/vrf.2021.521500.3135. Epub 2022 Sep 15.
Diagnosis of bovine viral diarrhea (BVD) relies on the detection of antibodies against its viral causing agent, bovine viral diarrhea virus (BVDV). Here, we designed a novel competitive ELISA (cELISA) using the most immunogenic part of BVDV nonstructural protein 3 (NS), as a single ELISA recombinant antigen, along with a monoclonal antibody to detect antibodies against BVDV in sera of infected animals. Hence, 197 serum samples were tested by this cELISA and the results were compared to the results obtained from virus neutralization test (VNT) as the gold standard method for diagnosis of BVD. McNemar's test indicated that there was no significant difference between the results of this newly designed cELISA and VNT. Meanwhile, kappa coefficients showed that there was a high correlation between these two assays. The relative sensitivity and specificity of cELISA with respect to VNT were 93.90% and 100%, respectively, suggesting that this newly designed cELISA could be a useful diagnostic tool for detection of BVDV infection. Moreover, as NS is highly conserved among Pestiviruses and the developed ELISA is a competitive one, it could potentially be applied to detect BVDV infection in other domestic and wildlife species.
牛病毒性腹泻（BVD）的诊断依赖于针对其致病病毒——牛病毒性腹泻病毒（BVDV）的抗体检测。在此，我们设计了一种新型竞争酶联免疫吸附测定法（cELISA），使用BVDV非结构蛋白3（NS）最具免疫原性的部分作为单一酶联免疫吸附测定重组抗原，以及一种单克隆抗体来检测感染动物血清中针对BVDV的抗体。因此，用这种cELISA对197份血清样本进行了检测，并将结果与作为BVD诊断金标准方法的病毒中和试验（VNT）所得结果进行了比较。McNemar检验表明，这种新设计的cELISA结果与VNT结果之间没有显著差异。同时，kappa系数表明这两种检测方法之间存在高度相关性。相对于VNT，cELISA的相对敏感性和特异性分别为93.90%和100%，这表明这种新设计的cELISA可能是检测BVDV感染的一种有用诊断工具。此外，由于NS在瘟病毒中高度保守，且所开发的酶联免疫吸附测定法是一种竞争法，它有可能应用于检测其他家养和野生动物物种中的BVDV感染。
