Duan Hao, He Fei, Wang Weizhou, Wang Yanghao, Yang Chenli, Lin Yihan, Li Ming, Lu You
Department of Orthopedics, The First Affiliated Hospital of Kunming Medical University, Kunming Yunnan, China.
Medical School, Kunming Medical University, Kunming, Yunnan, China.
Vet Res Forum. 2022 Sep;13(3):309-313. doi: 10.30466/vrf.2021.138754.3087. Epub 2022 Sep 15.
Elevated blood glucose concentration due to food intake will trigger insulin secretion from the dorsal pancreas has been extensively studied. This increased intracellular insulin level can stimulate glucagon release from intra-islets. However, the interaction between glucagon and insulin under a fasting state is unknown. To explore the relationship, we partially removed the ventral and dorsal pancreas on wild-type adult rats. The glucose tolerance test was conducted to measure influence of the surgery on the integrity function of the pancreas. The fasting insulin/glucagon level before and after surgery were measured by the ELISA kit. The statistical analyses indicated that the ventral removal of the pancreas had higher fasting glucose than that of dorsal removal. And only the ventral removal group showed significantly increased basal insulin and basal glucagon levels. Our findings showed differential role of the ventral pancreas in response to a glucose-free stimulus and also provided the possible target for developing the anti-hyperglycemic drugs.
因食物摄入导致的血糖浓度升高会触发背侧胰腺分泌胰岛素，这一过程已得到广泛研究。细胞内胰岛素水平的升高会刺激胰岛内胰高血糖素的释放。然而，禁食状态下胰高血糖素与胰岛素之间的相互作用尚不清楚。为了探究这种关系，我们对野生型成年大鼠部分切除了腹侧和背侧胰腺。进行葡萄糖耐量试验以测量手术对胰腺完整功能的影响。通过酶联免疫吸附测定试剂盒测量手术前后的空腹胰岛素/胰高血糖素水平。统计分析表明，腹侧胰腺切除组的空腹血糖高于背侧胰腺切除组。并且只有腹侧胰腺切除组的基础胰岛素和基础胰高血糖素水平显著升高。我们的研究结果显示了腹侧胰腺在应对无葡萄糖刺激时的不同作用，也为开发抗高血糖药物提供了可能的靶点。
