Mîndrilă Bogdan, Rogoveanu Ion, Buteică Sandra-Alice, Cercelaru Liliana, Mihaiescu Dan-Eduard, Mănescu Marina-Daniela, Mîndrilă Ion, Pirici Ionica
Doctoral School, University of Medicine and Pharmacy of Craiova, Romania.
Department of Gastroenterology, Faculty of Medicine University of Medicine and Pharmacy of Craiova, Romania.
Curr Health Sci J. 2022 Apr-Jun;48(2):146-154. doi: 10.12865/CHSJ.48.02.02. Epub 2022 Jun 30.
With a simple synthesis and easy engineering of physicochemical properties, iron oxide nanoparticles (IONPs) have become widely used in multiple biomedical applications. The study of IONPs toxicity has become an important issue, especially as the results reported so far are contradictory and range from lack of toxicity to cellular toxicity. The aim of this study was to evaluate the histopathological changes induced in mouse liver by long-term intraperitoneal injection of low doses of IONPs functionalized with salicylic acid (SaIONPs). The study was performed on C57BL/6 mice that received by intraperitoneal injection (IP), every two days, 0.6ml of SaIONPs aqueous suspension (35mg/kg body weight SaIONPs that contained 20mg/kg body weight of Fe3O4) for 28 days. The results of this study showed that the cumulative dose of 105mg/kg body weight SaIONPs (62mg/kg body weight of Fe3O4) induced histopathological changes in the subcapsular region of the mouse liver, possible by the release of salicylic acid into the peritoneal cavity. The cumulative dose of 244mg/kg body weight SaIONPs (145mg/kg body weight of Fe3O4) induced liver centrilobular necrosis, which requires the use of lower doses in biological applications. However, this may prove to be beneficial in the case of targeted accumulation of SaIONPs.
凭借简单的合成方法和易于调控的物理化学性质，氧化铁纳米颗粒（IONPs）已广泛应用于多种生物医学领域。IONPs的毒性研究已成为一个重要问题，特别是因为目前报道的结果相互矛盾，从无毒性到细胞毒性不等。本研究的目的是评估长期腹腔注射低剂量水杨酸功能化的IONPs（SaIONPs）对小鼠肝脏诱导的组织病理学变化。该研究在C57BL/6小鼠上进行，每两天腹腔注射（IP）0.6ml SaIONPs水悬浮液（35mg/kg体重的SaIONPs，含20mg/kg体重的Fe3O4），持续28天。本研究结果表明，105mg/kg体重的SaIONPs（62mg/kg体重的Fe3O4）累积剂量在小鼠肝脏被膜下区域诱导了组织病理学变化，可能是由于水杨酸释放到腹腔所致。244mg/kg体重的SaIONPs（145mg/kg体重的Fe3O4）累积剂量诱导了肝小叶中心坏死，这在生物应用中需要使用更低剂量。然而，在SaIONPs靶向积累的情况下，这可能被证明是有益的。
