CYP2B6 等位基因在主要伊朗族群中的频率，影响依非韦伦的反应。
Frequency of CYP2B6 Alleles in Major Iranian Ethnicities, Affecting Response to Efavirenz.
机构信息
Student Research Committee, Alborz University of Medical Sciences, Karaj, Iran.
Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran.
出版信息
Genet Res (Camb). 2022 Oct 19;2022:5754776. doi: 10.1155/2022/5754776. eCollection 2022.
INTRODUCTION
Efavirenz is an antihuman immunodeficiency virus (HIV) drug metabolized by cytochrome P450 2B6 (CYP2B6) enzyme. Cytochrome P450 2B6 is an enzyme that in humans is encoded by the CYP2B6 gene. Polymorphisms of this gene play a crucial role in the metabolism of drugs such as Efavirenz. This study aims to evaluate the frequency of three clinically significant CYP2B6 polymorphisms (CYP2B6 6 (516G > T), CYP2B6 4 (785A > G), and CYP2B6 5 (1459C > T)) in three major Iranian ethnicities.
METHODS
One hundred forty-seven participants from three main Iranian ethnicities were included in this study. After DNA extraction, CYP2B6 6 (516G > T), CYP2B6 4 (785A > G), and CYP2B6 5 (1459C > T) were genotyped using tetra-primer amplification refractory mutation system polymerase chain reaction (ARMS-PCR).
RESULTS
The frequency of the mutated allele in the Iranian population for CYP2B6 6 (516G > T) was 41.50 (95% CI: 35.81, 47.36), which was significantly lower than in Kurds (59.62, 95% CI: 45.10, 72.99). Similarly, Kurds had a higher frequency of mutated allele of CYP2B6 5 (1459C > T) (46.15%, 95% CI: 32.23, 60.53) than in Iranians (24.49%, 95% CI: 19.68, 29.82). The frequency of A and G alleles of CYP2B6 4 (785A > G) was 62.59% (95% CI: 56.78, 68.13) and 37.41 (95% CI: 31.87, 43.22), respectively.
CONCLUSION
Kurds are at higher risk of adverse drug reactions (ADRs) and insufficient anti-HIV response compared to other Iranians.
介绍
依非韦伦是一种抗人类免疫缺陷病毒（HIV）药物，由细胞色素 P450 2B6（CYP2B6）酶代谢。细胞色素 P450 2B6 是一种在人类中由 CYP2B6 基因编码的酶。该基因的多态性在依非韦伦等药物的代谢中起着至关重要的作用。本研究旨在评估三种具有临床意义的 CYP2B6 多态性（CYP2B6 6（516G>T）、CYP2B6 4（785A>G）和 CYP2B6 5（1459C>T））在三个主要伊朗种族中的频率。
方法
本研究纳入了来自三个主要伊朗民族的 147 名参与者。在提取 DNA 后，使用四引物扩增受阻突变系统聚合酶链反应（ARMS-PCR）对 CYP2B6 6（516G>T）、CYP2B6 4（785A>G）和 CYP2B6 5（1459C>T）进行基因分型。
结果
伊朗人群 CYP2B6 6（516G>T）突变等位基因的频率为 41.50%（95%CI：35.81，47.36），明显低于库尔德人（59.62%，95%CI：45.10，72.99%）。同样，库尔德人 CYP2B6 5（1459C>T）突变等位基因的频率也高于伊朗人（46.15%，95%CI：32.23，60.53%）（46.15%，95%CI：32.23，60.53%）。CYP2B6 4（785A>G）的 A 和 G 等位基因的频率分别为 62.59%（95%CI：56.78，68.13%）和 37.41%（95%CI：31.87，43.22%）。
结论
与其他伊朗人相比，库尔德人发生药物不良反应（ADR）和抗 HIV 反应不足的风险更高。
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