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一种基于荧光开启型DNA模板化银纳米簇的无标记ATP适配体传感器。

A label-free aptasensor for the detection of ATP based on turn-on fluorescence DNA-templated silver nanoclusters.

作者信息

Zhang Baozhu, Yang Ziyao, Li Yuxia, Ma Ling, Li Fenfang, Lv Xiuqing, Wen Guangming

机构信息

Department of Chemistry and Chemical Engineering, Jinzhong University Yuci 030619 P. R. China

出版信息

RSC Adv. 2022 Oct 20;12(46):30024-30029. doi: 10.1039/d2ra04636a. eCollection 2022 Oct 17.

Abstract

A label-free aptasensor has been fabricated in order to detect adenosine triphosphate (ATP) using turn-on fluorescence DNA-Ag NCs. The fluorescence of the DNA-Ag NCs could increase remarkably with the addition of ATP mainly because ATP specifically interacts with its aptamer to change the microenvironment of the darkish DNA-Ag NCs located at one terminus or two termini due to the conformational alteration of the aptamer structure. The proposed sensor can detect ATP in a linear range of 6-27 mM with a good detection limit of 5.0 μM. Additionally, the proposed method succeeded in detecting ATP in fetal bovine serum.

摘要

为了使用开启型荧光DNA-银纳米簇检测三磷酸腺苷(ATP),已经制备了一种无标记适体传感器。DNA-银纳米簇的荧光会随着ATP的加入而显著增加,这主要是因为ATP与其适体特异性相互作用,由于适体结构的构象改变,从而改变了位于一个末端或两个末端的深色DNA-银纳米簇的微环境。所提出的传感器能够在6-27 mM的线性范围内检测ATP,并具有5.0 μM的良好检测限。此外,所提出的方法成功地在胎牛血清中检测到了ATP。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1802/9582908/3f4c3b77692b/d2ra04636a-s1.jpg

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