Yan Bowen, Jia Ting, Wang Zhengkun, Zhu Wanlong
Key Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of Yunnan Province Higher Institutes College, School of Life Sciences, Yunnan Normal University, Kunming, China.
Yunnan College of Business Management, Kunming, China.
Front Microbiol. 2022 Oct 17;13:1026841. doi: 10.3389/fmicb.2022.1026841. eCollection 2022.
In order to investigate the effects of different areas on intestinal bacterial diversity and body mass regulation in from Hengduan mountain regions, and to explore the community structure and diversity of intestinal microflora and their role in body mass regulation. was collected from five areas including Deqin (DQ), Xianggelila (XGLL), Lijiang (LJ), Jianchuan (JC), and Dali (DL), we used 16S rRNA sequencing technology combined with physiological and morphological methods to study the intestinal microbiota diversity, abundance and community structure of the intestinal bacteria in winter, and to explore the influence of geographical factors, physiological indicators including food intake, resting metabolic rate (RMR), non-shivering thermogenesis (NST), neuropeptide Y (NPY), Agouti-Related Protein (AgRP), proopiomelanocortin (POMC), cocaine and amphetamine regulated transcription peptide (CART), and morphological indicators including body mass, body length and other nine indicators on the intestinal microflora diversity in . The results showed that there were significant differences in metabolic indexes such as RMR, NST, NPY, AgRP, and morphological indexes such as body length, tail length and ear length among the five regions. Bacterial community in intestinal tract of mainly includes three phyla, of which Firmicutes is the dominant phyla, followed by Bacteroidetes and Tenericutes. At the genus level, the dominant bacterial genera were S24-7(UG), Clostridiales (UG), and Lachnospiraceae (UG), etc. α diversity of intestinal microorganisms in DL and JC were significantly different from that in the other three regions. Genera of intestinal microorganisms in DL and JC were also the most. Moreover, , , and could affect energy metabolism in , which were closely related to the environment in which they lived. All of these results indicated that different areas in Hengduan Mountain had certain effects on the structure of intestinal microbial community in , which were responded positively to changes in food abundance and other environmental factors. Furthermore, Firmicutes and Bacteroidetes play an important role in the body mass regulation in .
为了研究横断山区不同区域对 肠道细菌多样性和体重调节的影响，探索肠道微生物群落结构和多样性及其在体重调节中的作用。从德钦（DQ）、香格里拉（XGLL）、丽江（LJ）、剑川（JC）和大理（DL）五个地区采集 ，采用16S rRNA测序技术结合生理和形态学方法，研究冬季肠道细菌的微生物群多样性、丰度和群落结构，探讨地理因素、包括食物摄入量、静息代谢率（RMR）、非颤抖产热（NST）、神经肽Y（NPY）、刺鼠相关蛋白（AgRP）、阿黑皮素原（POMC）、可卡因和安非他明调节转录肽（CART）等生理指标，以及包括体重、体长等九个指标的形态学指标对 肠道微生物群多样性的影响。结果表明，五个地区间RMR、NST、NPY、AgRP等代谢指标以及体长、尾长、耳长等形态指标存在显著差异。 肠道细菌群落主要包括三个门，其中厚壁菌门是优势门，其次是拟杆菌门和柔膜菌门。在属水平上，优势细菌属为S24-7（UG）、梭菌目（UG）、毛螺菌科（UG）等。DL和JC的肠道微生物α多样性与其他三个地区显著不同。DL和JC的肠道微生物属也最多。此外， 、 和 可影响 中的能量代谢，这与它们生活的环境密切相关。所有这些结果表明，横断山不同区域对 肠道微生物群落结构有一定影响，它们对食物丰度等环境因素的变化呈积极响应。此外，厚壁菌门和拟杆菌门在 体重调节中起重要作用。
