College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; College of Agriculture and Forestry Science, Linyi University, Linyi 276005, People's Republic of China.
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Department of Pathobiology, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, USA.
Microb Pathog. 2017 Oct;111:60-70. doi: 10.1016/j.micpath.2017.08.025. Epub 2017 Aug 18.
Diarrhea, the most common disease of perinatal yaks greatly affects the growth of animals. Changes in the number and structure of intestinal flora can cause the disorder of the intestinal environment leading to diarrhea. A study was conducted to investigate the impact of diarrhea on the number and structure of intestinal flora in perinatal yaks. Fecal samples were collected from healthy and diarrhea-affected perinatal yaks; and changes in number and structure of intestinal flora were compared by 16S rDNA V3-V4 region high-throughput sequencing. A total of 272071 optimized sequences were acquired from bacteria, which were identified from 9 phyla, 13 classes, 17 orders, 36 families and 72 genera. The number of bacterial species among diarrheac intestinal flora was lower than the healthy group, with no significant difference between two groups (P > 0.05); however, significant differences were observed at phylum, class, order, family and genus level between two groups (P < 0.05). This study has provided for the first time an insight of the changes occurring in intestinal flora in perinatal yaks at high altitudes of the world.
腹泻是围产期牦牛最常见的疾病，极大地影响了动物的生长。肠道菌群数量和结构的变化会导致肠道环境紊乱，从而引发腹泻。本研究旨在探讨腹泻对围产期牦牛肠道菌群数量和结构的影响。采集健康和腹泻牦牛的粪便样本，通过 16S rDNA V3-V4 区高通量测序比较肠道菌群数量和结构的变化。从细菌中获得了 272071 条优化序列，这些细菌可分为 9 个门、13 个纲、17 个目、36 个科和 72 个属。腹泻组肠道菌群的细菌种类数量低于健康组，但两组间无显著性差异（P>0.05）；然而，两组间在门、纲、目、科和属水平上均有显著差异（P<0.05）。本研究首次提供了世界高海拔地区围产期牦牛肠道菌群变化的见解。
