Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, China.
Pharm Biol. 2020 Dec;58(1):636-645. doi: 10.1080/13880209.2020.1784234.
extracts (FMTE) have a good therapeutic effect on coccidiosis, but there is no relevant research on its prophylactic effect on coccidiosis.
This study comprehensively evaluates the anticoccidial effect of FMTE.
, the unsporulated oocysts were treated with serial dilutions of FMTE and incubated for 7 d, and the sporulated oocysts were counted for calculating the median lethal concentration (LC) of FMTE. , 180 10-day-old broiler chickens free of coccidiosis were weighted and randomly distributed into six groups: normal group, untreated group, 4 protective groups (positive group and three FMTE groups). From day 10 to day 21, chickens in the three FMTE groups were pre-treated with FMTE at the dosage of 2.5, 5 and 10 g/kg/d, respectively, and chickens in the positive group were pre-treated with qiuliling (10 g/kg/d). On day 14, chickens in all groups except the normal group were orally infected with 1.5 × 10 sporulated oocysts. The clinical symptoms were observed from day 10 to day 21, the anticoccidial index (ACI), tissue lesions, and intestinal microflora were determined on day 21.
FMTE showed anti-sporulation effect against and the LC value was 245.83 µg/mL . , FMTE at the dosage of 10 g/kg/d was effective against infection, and its ACI value was 162.56, which was higher than the value of positive drug qiuliling (128.81). FMTE have potent anticoccidial effects, and it presents an alternative anticoccidial agent for avian coccidiosis control.
提取物（FMTE）对球虫病有很好的治疗效果，但关于其对球虫病的预防作用尚无相关研究。
本研究综合评价 FMTE 的抗球虫效果。
采用连续稀释 FMTE 处理未孢子化卵囊并孵育 7d，计数孢子化卵囊以计算 FMTE 的半数致死浓度（LC）。选择 180 只 10 日龄无球虫感染的肉鸡，称重并随机分为 6 组：正常组、未处理组、4 个保护组（阳性组和 3 个 FMTE 组）。从第 10 天到第 21 天，3 个 FMTE 组的鸡分别用 2.5、5 和 10g/kg/d 的剂量进行预治疗，阳性组的鸡用秋丽灵（10g/kg/d）进行预治疗。第 14 天，除正常组外，所有组的鸡均口服感染 1.5×10 个孢子化卵囊。从第 10 天到第 21 天观察临床症状，第 21 天测定抗球虫指数（ACI）、组织病变和肠道微生物群。
FMTE 对 和显示抗孢子化作用，LC 值为 245.83µg/mL。FMTE 在 10g/kg/d 剂量下对 感染有效，其 ACI 值为 162.56，高于阳性药物秋丽灵（128.81）。FMTE 具有很强的抗球虫作用，为禽球虫病防治提供了一种替代抗球虫药物。
