Department of Medical Biochemistry, Faculty of Medicine, Firat University, Elazıg, Turkey.
Department of Medical Pathology, Faculty of Medicine, Firat University, Elazıg, Turkey.
J Biochem Mol Toxicol. 2019 Sep;33(9):e22371. doi: 10.1002/jbt.22371. Epub 2019 Jul 23.
Breast cancer is the most common cancer among women in the world and the incidence is increasing alarmingly. It was aimed to determine the effect of raloxifene (RAL) and fluoxetine (FLX) on selected parameters in 7,12-dimethylbenz(a)anthracene (DMBA)-induced mammary carcinoma. Thirty-two female Wistar albino rats were assorted into four groups: DMBA (group I), DMBA+RAL (group II), DMBA+FLX (group III), and DMBA+RAL+FLX (group IV). Mammary tissue vascular endothelial growth factor (VEGF), macrophage colony-stimulating factor (M-CSF), matrix metalloproteinase-9 (MMP-9), and tissue inhibitors of matrix metalloproteinase-1 (TIMP-1) levels were determined by the enzyme-linked immunosorbent assay method. The tissue VEGF levels were lower in group IV compared with DMBA group. Decreased M-CSF levels were observed in all therapeutic groups rather than the DMBA group, but the most effective decrease was found in group IV. Compared with the DMBA group, MMP-9 levels were statistically significantly decreased in group II and group IV. However, TIMP-1 levels were higher in the whole therapeutic groups rather than the DMBA group and the most effective increase was observed in group IV. Results of the present study suggest that combined therapy of RAL with FLX might lead to a better outcome targeting breast tumor.
乳腺癌是全世界女性中最常见的癌症，发病率令人震惊地上升。本研究旨在探讨雷洛昔芬（RAL）和氟西汀（FLX）对 7,12-二甲基苯并蒽（DMBA）诱导的乳腺癌中某些参数的影响。将 32 只雌性 Wistar 白化大鼠分为四组：DMBA（第 I 组）、DMBA+RAL（第 II 组）、DMBA+FLX（第 III 组）和 DMBA+RAL+FLX（第 IV 组）。采用酶联免疫吸附试验法测定乳腺组织血管内皮生长因子（VEGF）、巨噬细胞集落刺激因子（M-CSF）、基质金属蛋白酶-9（MMP-9）和基质金属蛋白酶组织抑制剂-1（TIMP-1）水平。与 DMBA 组相比，第 IV 组组织 VEGF 水平较低。所有治疗组的 M-CSF 水平均低于 DMBA 组，但第 IV 组下降最明显。与 DMBA 组相比，第 II 组和第 IV 组 MMP-9 水平显著降低。然而，整个治疗组的 TIMP-1 水平均高于 DMBA 组，第 IV 组升高最明显。本研究结果表明，RAL 联合 FLX 治疗可能会对乳腺癌产生更好的疗效。
