Vilela Diego Júnior Martins, Pinto Renan Terassi, Cardoso Thiago Bérgamo, Paiva Luciano Vilela, Carneiro Marco Aurélio Carbone, Carvalho Gladyston Rodrigues, Dos Santos Jessé Valentim
Agricultural Research Company of Minas Gerais (Epamig), Epamig Oeste, Patrocínio, MG Brazil.
Laboratory of Molecular Biology, Department of Chemistry, Federal University of Lavras, Lavras, MG Brazil.
3 Biotech. 2022 Dec;12(12):335. doi: 10.1007/s13205-022-03399-6. Epub 2022 Nov 1.
Phosphorus (P) availability is determinant for crop productivity and, despite the sufficient amount of this nutrient in arable land, most of it remains insoluble, leading to the need of high fertilizer input. To cope with P scarcity forecasts and also for cropping costs alleviation, genotypes better adapted to promote P solubilization and absorption are required, especially for perennial crops. Coffee is one of these important perennial crops cultivated in soils with low P availability, and thus we aimed to study adaptive strategies to coffee genotypes in acquire phosphorus. In this study, we focused on rhizosphere phosphatase activity, a major characteristic related to P solubilization from organic pools. To explore the genetic basis of this characteristic, we firstly identified 29 () genes in genome and selected five candidates with higher potential to encode secreted PAPs. We found that genotypes have diverse profiles of rhizosphere phosphatase activity, as well as microbial biomass carbon, which we measured to explore the impact of the plant on microbiota and also to discriminate the phosphatase activity origin (plant or microorganism-derived). We selected two cultivars with contrasting phosphatase activity and found that one gene has a correlated gene expression profile with this characteristic. This work explores coffee adaptative responses to P starvation conditions, and the information provided can further contribute to breeding programs aiming better adapted genotypes for sustainable agriculture in face of current challenges.
The online version contains supplementary material available at 10.1007/s13205-022-03399-6.
磷（P）的有效性是作物生产力的决定因素，尽管耕地中这种养分含量充足，但大部分仍不溶解，导致需要大量施肥。为应对磷短缺预测并减轻种植成本，需要更能促进磷溶解和吸收的基因型，特别是对于多年生作物。咖啡是在低磷土壤中种植的重要多年生作物之一，因此我们旨在研究咖啡基因型获取磷的适应策略。在本研究中，我们关注根际磷酸酶活性，这是与从有机库中溶解磷相关的主要特征。为探索这一特征的遗传基础，我们首先在基因组中鉴定出29个（）基因，并选择了五个编码分泌型酸性磷酸酶（PAPs）潜力较高的候选基因。我们发现咖啡基因型具有不同的根际磷酸酶活性以及微生物生物量碳特征，并对其进行了测量，以探究植物对微生物群的影响，并区分磷酸酶活性的来源（植物或微生物来源）。我们选择了两个磷酸酶活性相反的咖啡品种，发现一个基因与该特征具有相关的基因表达谱。这项工作探索了咖啡对磷饥饿条件的适应性反应，所提供的信息可为育种计划提供进一步帮助，以培育出更适应可持续农业当前挑战的基因型。
在线版本包含可在10.1007/s132-05-022-03399-6获取的补充材料。
