Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University, Seoul, South Korea.
Innovation Research and Support Center for Dental Science, Yonsei University Dental Hospital, Seoul, South Korea.
J Clin Periodontol. 2023 Mar;50(3):358-367. doi: 10.1111/jcpe.13742. Epub 2022 Nov 25.
It has been proposed that platelet-rich fibrin (PRF) can be used to support bone regeneration during alveolar ridge augmentation. The aim of this study was to determine whether an approach utilizing PRF provides similar performance to the established guided bone regeneration (GBR) procedure.
Two-wall defects were surgically created in beagle dogs and treated in three experimental groups: (i) a sticky bone (SB) substitute prepared using liquid PRF and deproteinized porcine bone mineral (DPBM); (ii) SB covered with solid PRF compressed into a membrane; and (iii) GBR performed using DPBM covered by a collagen membrane. Quantitative reverse-transcription polymerase chain reaction was applied to the specimen after 1 week of healing, and microcomputed tomography (micro-CT) and histological outcomes were analysed after 8 weeks of healing.
Compared with GBR, PRF resulted in a moderate increase in the expression levels of osteoblast and osteoclast markers, osteocalcin, and calcitonin receptor. Moreover, PRF modestly increased angiogenesis and the inflammation markers vascular endothelial growth factor (VEGF) and IL-6. Micro-CT and histological analyses confirmed the expected increased alveolar ridge area, with no significant differences between the three groups. Consistently, graft consolidation, as indicated by new bone formation at the defect site, did not differ significantly between groups.
The present results demonstrate that PRF-based approaches perform comparably to the established GBR procedure in terms of the consolidation of DPBM in two-wall alveolar defects.
有人提出，富血小板纤维蛋白（PRF）可用于支持牙槽嵴增高术中的骨再生。本研究旨在确定利用 PRF 的方法是否与已建立的引导骨再生（GBR）程序具有相似的性能。
在比格犬中手术创建双壁缺损，并在三个实验组中进行治疗：（i）使用液体 PRF 和去蛋白猪骨矿物质（DPBM）制备的粘性骨（SB）替代物；（ii）用压缩成膜的固体 PRF 覆盖的 SB；（iii）用 DPBM 覆盖胶原膜的 GBR。在愈合后 1 周应用定量逆转录聚合酶链反应对标本进行分析，在愈合后 8 周进行 micro-CT 和组织学分析。
与 GBR 相比，PRF 导致成骨细胞和破骨细胞标志物、骨钙素和降钙素受体的表达水平适度增加。此外，PRF 适度增加了血管生成和炎症标志物血管内皮生长因子（VEGF）和白细胞介素 6（IL-6）。micro-CT 和组织学分析证实了预期的牙槽嵴面积增加，三组之间无显著差异。一致地，在缺陷部位新骨形成所表示的移植物整合方面，各组之间没有显著差异。
本研究结果表明，在双壁牙槽缺损中 DPBM 整合方面，基于 PRF 的方法与已建立的 GBR 程序性能相当。
