Applied Entomology and Medical Toxicology Laboratory, Department of Zoology, Government College University, Lahore, Pakistan.
Department of Zoology, Women University of Azad Jammu Kashmir, Bagh, Pakistan.
J Burn Care Res. 2023 Jul 5;44(4):800-809. doi: 10.1093/jbcr/irac169.
Diabetes is involved in delayed wound healing that can be cured by natural products such as garlic, turmeric, and fibroin extracts. Alloxan monohydrate is used for inducing diabetes in mice. The percent wound contraction of garlic (150 mg/ml), turmeric (100 mg/ml), and fibroin (50 mg/ml), individually and in combinations garlic (150 mg/ml) + fibroin (50 mg/ml), turmeric (100 mg/ml) + fibroin (50 mg/ml), garlic (150 mg/ml) + turmeric (100 mg/ml), and garlic (150 mg/ml) + turmeric (100 mg/ml) + fibroin (50 mg/ml) was checked by evaluating the healing time, % wound contraction and histological analysis. The serum level of MMPs (MMP 2, MMP7, MMP 9), pro-inflammatory cytokines (TNF-α, IL-6, IL-8), and TIMPs were evaluated. With the combination of three extracts (Ga+Tu+Fi) garlic (150 mg/ml), turmeric (100 mg/ml) and fibroin (50 mg/ml), wounds healed in 12 days and had 97.3 ± 2.2% wound contraction. While the positive control (polyfax) and diabetic control (saline) wounds healed in 17- and 19-days with wound contraction of 96.7 ± 1.4% and 96.3 ± 1.1%, respectively. Histological analysis showed that the combination of Ga+Tu+Fi exhibited an increase in the growth of collagen fibers, fibroblasts number, and keratinocytes, and lessened inflammation of blood vessels. The combination of Ga+Tu+Fi significantly alleviated the serum concentration of TNF-α (14.2 ± 0.7 pg/ml), IL-6 (10.0 ± 1.0 pg/ml), IL-8 (16.0 ± 1.5 pg/ml), MMP2 (228.0 ± 18.1 pg/ml), MMP7 (271.0 ± 9.9 pg/ml), and MMP9 (141.0 ± 5.3 pg/ml) to diabetic control. The level of TIMPs (193.0 ± 9.1 pg/ml) was increased significantly with respect to diabetic control. We conclude that the combination of these biomaterials possessed high regenerative and healing capabilities and can be an effective remedy in the healing of chronic wounds in diabetic patients.
糖尿病会导致伤口愈合延迟，而某些天然产物，如大蒜、姜黄和丝素提取物，可对此进行治疗。一水合盐酸用于诱导小鼠的糖尿病。通过评估愈合时间、%伤口收缩率和组织学分析，检查了大蒜（150mg/ml）、姜黄（100mg/ml）和丝素（50mg/ml）单独和联合使用（大蒜（150mg/ml）+丝素（50mg/ml）、姜黄（100mg/ml）+丝素（50mg/ml）、大蒜（150mg/ml）+姜黄（100mg/ml）和大蒜（150mg/ml）+姜黄（100mg/ml）+丝素（50mg/ml））对伤口收缩的影响。还评估了血清中基质金属蛋白酶（MMP2、MMP7、MMP9）、促炎细胞因子（TNF-α、IL-6、IL-8）和 TIMPs 的水平。三种提取物（大蒜+姜黄+丝素）联合使用（大蒜 150mg/ml、姜黄 100mg/ml 和丝素 50mg/ml）时，伤口在 12 天内愈合，伤口收缩率为 97.3±2.2%。阳性对照（多福来）和糖尿病对照（生理盐水）伤口分别在 17 天和 19 天愈合，伤口收缩率分别为 96.7±1.4%和 96.3±1.1%。组织学分析表明，大蒜+姜黄+丝素联合使用可增加胶原蛋白纤维、成纤维细胞数量和角质形成细胞的生长，并减少血管炎症。大蒜+姜黄+丝素联合使用可显著降低 TNF-α（14.2±0.7pg/ml）、IL-6（10.0±1.0pg/ml）、IL-8（16.0±1.5pg/ml）、MMP2（228.0±18.1pg/ml）、MMP7（271.0±9.9pg/ml）和 MMP9（141.0±5.3pg/ml）在糖尿病对照组中的血清浓度。与糖尿病对照组相比，TIMP 水平（193.0±9.1pg/ml）显著升高。我们得出结论，这些生物材料的组合具有较高的再生和愈合能力，可有效治疗糖尿病患者的慢性伤口。
