来源于印度的儿茶素、表儿茶素、姜黄素、大蒜、石榴皮和印楝提取物通过降低 TGF-β 和 TNF-α 的表达，显示出增强人原代急性和慢性伤口衍生成纤维细胞的抗炎潜力。
Catechin, epicatechin, curcumin, garlic, pomegranate peel and neem extracts of Indian origin showed enhanced anti-inflammatory potential in human primary acute and chronic wound derived fibroblasts by decreasing TGF-β and TNF-α expression.
机构信息
Department of Biotechnology, M.S. Ramaiah Institute of Technology, MSR Nagar, MSRIT Post, Bangalore, 560054, India.
Visvesvaraya Technological University, Jnana Sangama, Belgaum, 590018, India.
出版信息
BMC Complement Med Ther. 2023 Jun 2;23(1):181. doi: 10.1186/s12906-023-03993-y.
BACKGROUND
Although chronic wounds are devastating and can cause burden at multiple levels, chronic wound research is still far behind. Chronic wound treatment is often less efficient due to delay in diagnosis and treatment, non-specific treatment mainly due to lack of knowledge of wound healing or healing resistance genes. It's known that chronic wounds do not progress towards healing, because it gets stalled in inflammatory phase of wound healing.
OBJECTIVE
We aimed to use phytoextracts possessing excellent anti-inflammatory properties to regulate the unbalanced levels of cytokines responsible for increased inflammation.
METHODS
Evaluation of anti-inflammatory activity of selected phytoextracts namely, Camellia sinensis (L.) Kuntze, Acacia catechu (L.f) Willd., Curcuma longa (L.), Allium sativum (L.), Punica granatum (L.) and Azadirachta indica A. hereafter, called as catechin, epicatechin, curcumin, garlic, pomegranate and neem extracts, respectively in Acute wound fibroblasts (AWFs) and Chronic wound fibroblasts (CWFs) using flow cytometry.
RESULTS
The phytoextracts exhibited no cytotoxicity below 100 μg/ml on normal Human Dermal fibroblasts (HDFs), while garlic extract showed highest cell viability followed by catechin, epicatechin, curcumin, pomegranate peel and neem based on IC value. Garlic, catechin and epicatechin extracts showed highest anti-inflammatory activities for both TGF-β and TNF-α in both AWFs and CWFs treated cells. After treatment of AWFs with catechin, epicatechin and garlic extracts, TGF-β and TNF-α expression was significantly reduced compared to untreated AWFs and reached to almost normal HDFs level. Also, after treatment of CWFs with catechin, epicatechin and garlic extracts, TGF-β and TNF-α expression was significantly reduced compared to untreated CWFs and was lesser than untreated AWFs.
CONCLUSION
The present findings reveal the potential of catechin, epicatechin and garlic extracts for the treatment of acute and chronic wounds with excellent anti-inflammatory properties.
背景
尽管慢性伤口具有破坏性，并可能在多个层面造成负担，但慢性伤口研究仍远远落后。由于诊断和治疗的延误，慢性伤口的治疗往往效率较低，而且由于缺乏对伤口愈合或愈合抵抗基因的了解，通常采用非特异性治疗。众所周知，慢性伤口不会朝着愈合的方向发展，因为它在伤口愈合的炎症期停滞不前。
目的
我们旨在使用具有优异抗炎特性的植物提取物来调节导致炎症增加的细胞因子的失衡水平。
方法
使用流式细胞术评估选定的植物提取物（即儿茶素、金合欢、姜黄、大蒜、石榴和印楝）的抗炎活性，分别称为儿茶素、表儿茶素、姜黄素、大蒜、石榴和印楝提取物，在急性伤口成纤维细胞（AWFs）和慢性伤口成纤维细胞（CWFs）中进行评估。
结果
在低于 100μg/ml 的浓度下，植物提取物对正常人类皮肤成纤维细胞（HDFs）无细胞毒性，而大蒜提取物显示出最高的细胞活力，其次是儿茶素、表儿茶素、姜黄素、石榴皮和印楝。大蒜、儿茶素和表儿茶素提取物在 AWFs 和 CWFs 处理的细胞中对 TGF-β 和 TNF-α 均表现出最高的抗炎活性。在用儿茶素、表儿茶素和大蒜提取物处理 AWFs 后，与未经处理的 AWFs 相比，TGF-β 和 TNF-α 的表达显著降低，达到接近正常 HDFs 的水平。此外，在用儿茶素、表儿茶素和大蒜提取物处理 CWFs 后，与未经处理的 CWFs 相比，TGF-β 和 TNF-α 的表达显著降低，且低于未经处理的 AWFs。
结论
本研究结果表明，儿茶素、表儿茶素和大蒜提取物具有治疗急性和慢性伤口的潜力，具有优异的抗炎特性。
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