Research Center for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
Research Center for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
Ecotoxicol Environ Saf. 2022 Dec 1;247:114252. doi: 10.1016/j.ecoenv.2022.114252. Epub 2022 Nov 1.
T-2 toxin belongs to the trichothecenes group A compound, mainly produced by Fusarium fungi. It has been shown that T-2 toxin could cross the placental barrier and breast milk, thus endangering the health of offspring. The present study aimed to explore the effects of maternal T-2 toxin exposure on the integrity of the intestinal barrier and the intestinal microflora of young mice. From late pregnancy (GD 14) to lactation (LD 21), pregnant mice were given T-2 toxin daily at 0, 0.005, or 0.05 mg T-2 toxin/kg BW. Postnatal day 21 (PND21), PND28, and PND56 young mice were chosen as objects to detect the influences of maternal T-2 toxin exposure to mice on the offspring. The results showed that maternal exposure to T-2 toxin disturbed the balance of the intestinal microbial flora of the young mice. Villous adhesions and fusion of ileum were observed in T-2-treated groups. In addition, supplementation of T-2 toxin significantly decreased the gene expressions of Claudin 1, Occludin, Tjp1, Il10, Il6, and Tnf in PND 21. However, in PND 28, the expressions of Tnf were significantly increased. The expressions of Claudin 1, Occludin, Tjp1, Il10, Il6 and Tnf were significantly increased after T-2 toxin treatment in PND 56. These results suggested that maternal exposure to T-2 toxin has negative influences on the intestine of young mice, which may be due to the alterations of microbial composition.
T-2 毒素属于 A 型单端孢霉烯族化合物，主要由镰刀菌产生。研究表明，T-2 毒素可以穿过胎盘屏障和母乳，从而危害后代的健康。本研究旨在探讨母体 T-2 毒素暴露对幼鼠肠道屏障完整性和肠道微生物群的影响。从妊娠晚期（GD 14）到哺乳期（LD 21），每天给怀孕的老鼠服用 0、0.005 或 0.05mg/kg BW 的 T-2 毒素。选择出生后第 21 天（PND21）、第 28 天（PND28）和第 56 天（PND56）的幼鼠作为研究对象，以检测母体 T-2 毒素暴露对幼鼠的影响。结果表明，母体暴露于 T-2 毒素会扰乱幼鼠肠道微生物菌群的平衡。在 T-2 处理组中观察到回肠绒毛粘连和融合。此外，T-2 毒素的补充显著降低了 PND21 时 Claudin 1、Occludin、Tjp1、Il10、Il6 和 Tnf 的基因表达。然而，在 PND28 时，Tnf 的表达显著增加。在 PND56 时，T-2 毒素处理后 Claudin 1、Occludin、Tjp1、Il10、Il6 和 Tnf 的表达显著增加。这些结果表明，母体暴露于 T-2 毒素对幼鼠的肠道有负面影响，这可能是由于微生物组成的改变所致。
