National Experimental Teaching Demonstration Center of Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, PR China.
National Experimental Teaching Demonstration Center of Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, PR China.
Ecotoxicol Environ Saf. 2020 Jun 15;196:110530. doi: 10.1016/j.ecoenv.2020.110530. Epub 2020 Mar 28.
T-2 toxin is a kind of group A trichothecenes mycotoxins, frequently detected in various foods and feeds, having high toxic effects on both humans and animals. The present study aims to investigate the toxic effects of T-2 toxin exposure to ICR mice during pregnancy and lactation on liver glycolipid metabolism of young mice. The pregnant mice were given 0, 0.005 and 0.05 mg of T-2 toxin/kg bw daily through oral gavage from late gestation (GD 14) to the lactation (LD 21). Liver and serum samples of the young mice were collected on postnatal day 21 (PND 21), PND 28 and PND 56. The results showed that T-2 toxin increased the contents of triglycerides (TG), total cholesterol (T-CHO) and glucose in serum of young mice on PND 21 and PND 28. In addition, obvious lipid droplets of liver in T-2 toxin treatment groups were observed, especially in 0.05 mg group of PND 21and PND 28. Compared with the control group, T-2 treatment also increased the expressions of genes associated with liver glycolipid metabolism, such as PEPCK, Glut2, Fas, Acox1, Hmgcr, PPARα, Srebp1 and CD36. These results demonstrated T-2 toxin exposure to pregnant mice could cause liver glycolipid metabolism disruption in the young mice and the toxic effects weakened on PND 56.
T-2 毒素是一种 A 型单端孢霉烯族真菌毒素，经常在各种食物和饲料中被检测到，对人类和动物都有很高的毒性作用。本研究旨在探讨 T-2 毒素暴露于妊娠和哺乳期 ICR 小鼠对幼鼠肝脏糖脂代谢的毒性作用。怀孕小鼠从妊娠后期（GD 14）到哺乳期（LD 21）每天通过口服灌胃给予 0、0.005 和 0.05mg/kg bw 的 T-2 毒素。在出生后第 21 天（PND 21）、第 28 天（PND 28）和第 56 天（PND 56）收集幼鼠的肝脏和血清样本。结果表明，T-2 毒素增加了 PND 21 和 PND 28 幼鼠血清中甘油三酯（TG）、总胆固醇（T-CHO）和葡萄糖的含量。此外，T-2 毒素处理组的肝脏中观察到明显的脂质滴，尤其是在 PND 21 和 PND 28 的 0.05mg 组。与对照组相比，T-2 处理还增加了与肝脏糖脂代谢相关的基因的表达，如 PEPCK、Glut2、Fas、Acox1、Hmgcr、PPARα、Srebp1 和 CD36。这些结果表明，妊娠小鼠暴露于 T-2 毒素可导致幼鼠肝脏糖脂代谢紊乱，且在 PND 56 时毒性作用减弱。
