Huijbers Mieke M E, Zhang Wuyuan, Tonin Fabio, Hollmann Frank
Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629, HZ, Delft, The Netherlands.
Angew Chem Int Ed Engl. 2018 Oct 8;57(41):13648-13651. doi: 10.1002/anie.201807119. Epub 2018 Sep 12.
The photoenzymatic decarboxylation of fatty acids to alkanes is proposed as an alternative approach for the synthesis of biodiesel. By using a recently discovered photodecarboxylase from Chlorella variabilis NC64A (CvFAP) we demonstrate the irreversible preparation of alkanes from fatty acids and triglycerides. Several fatty acids and their triglycerides are converted by CvFAP in near-quantitative yield and exclusive selectivity upon illumination with blue light. Very promising turnover numbers of up to 8000 were achieved in this proof-of-concept study.
脂肪酸光酶脱羧生成烷烃被提议作为合成生物柴油的一种替代方法。通过使用最近从小球藻NC64A（CvFAP）中发现的光脱羧酶，我们证明了从脂肪酸和甘油三酯不可逆地制备烷烃。几种脂肪酸及其甘油三酯在蓝光照射下被CvFAP以接近定量的产率和唯一的选择性进行转化。在这项概念验证研究中实现了高达8000的非常有前景的周转数。
