斑马鱼Bmp家族三个成员Bmp2a、Bmp2b和Bmp4的克隆与表达

Cloning and expression of three members of the zebrafish Bmp family: Bmp2a, Bmp2b and Bmp4.

作者信息

Martínez-Barberá J P, Toresson H, Da Rocha S, Krauss S

机构信息

University of Lund, Department of Cell and Molecular Biology, Sweden.

出版信息

Gene. 1997 Oct 1;198(1-2):53-9. doi: 10.1016/s0378-1119(97)00292-8.

DOI:10.1016/s0378-1119(97)00292-8

PMID:9370264

Abstract

In vertebrates, Bmps (bone morphogenetic proteins) play critical roles in establishing the basic embryonic body plan and are involved in the development of a large variety of organs and tissues. To study the evolution of Bmps, we isolated cDNAs for three members of the zebrafish Bmp gene family: Bmp2a, Bmp2b and Bmp4. The deduced amino acid sequences of Bmp2a and Bmp4 consist of 386 and 400 aa, respectively and show high homologies to their counterparts in mouse, chick and Xenopus. The deduced Bmp2b aa sequence consists of 411 aa and the mature protein shows 88% and 86% identities to zebrafish Bmp2a and Bmp4, respectively. The expression of the mRNA of these three genes has been analyzed by whole mount in situ hybridization and RT-PCR. Areas of zebrafish Bmp2 and Bmp4 expression suggest evolutionary conserved mechanisms of Bmp2/4 dependent differentiation between lower and higher vertebrates.

摘要

在脊椎动物中，骨形态发生蛋白（Bmps）在确立基本胚胎体轴过程中发挥关键作用，并参与多种器官和组织的发育。为研究Bmps的进化，我们分离出斑马鱼Bmp基因家族三个成员的cDNA：Bmp2a、Bmp2b和Bmp4。Bmp2a和Bmp4推导的氨基酸序列分别由386和400个氨基酸组成，与小鼠、鸡和非洲爪蟾中的对应序列具有高度同源性。推导的Bmp2b氨基酸序列由411个氨基酸组成，成熟蛋白与斑马鱼Bmp2a和Bmp4的同一性分别为88%和86%。已通过整体原位杂交和逆转录聚合酶链反应（RT-PCR）分析了这三个基因的mRNA表达情况。斑马鱼Bmp2和Bmp4的表达区域表明，低等和高等脊椎动物之间存在依赖Bmp2/4分化的进化保守机制。