使用单核苷酸表观突变分析鉴定长链非编码RNA：一种新型基因发现方法。
Identification of long non-coding RNA using single nucleotide epimutation analysis: a novel gene discovery approach.
作者信息
Kerachian Mohammad Amin, Azghandi Marjan
机构信息
Medical Genetics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
出版信息
Cancer Cell Int. 2022 Nov 4;22(1):337. doi: 10.1186/s12935-022-02752-2.
BACKGROUND
Long non-coding RNAs (lncRNAs) are involved in a variety of mechanisms related to tumorigenesis by functioning as oncogenes or tumor-suppressors or even harboring oncogenic and tumor-suppressing effects; representing a new class of cancer biomarkers and therapeutic targets. It is predicted that more than 35,000 ncRNA especially lncRNA are positioned at the intergenic regions of the human genome. Emerging research indicates that one of the key pathways controlling lncRNA expression and tissue specificity is epigenetic regulation.
METHODS
In the current article, a novel approach for lncRNA discovery based on the intergenic position of most lncRNAs and a single CpG site methylation level representing epigenetic characteristics has been suggested.
RESULTS
Using this method, a novel antisense lncRNA named LINC02892 presenting three transcripts without the capacity of coding a protein was found exhibiting nuclear, cytoplasmic, and exosome distributions.
CONCLUSION
The current discovery strategy could be applied to identify novel non-coding RNAs influenced by methylation aberrations.
背景
长链非编码RNA（lncRNAs）通过作为癌基因或肿瘤抑制因子发挥作用，甚至兼具致癌和抑癌作用，参与多种与肿瘤发生相关的机制；代表了一类新型的癌症生物标志物和治疗靶点。据预测，超过35,000种非编码RNA尤其是lncRNA位于人类基因组的基因间区域。新兴研究表明，控制lncRNA表达和组织特异性的关键途径之一是表观遗传调控。
方法
在本文中，基于大多数lncRNAs的基因间位置和代表表观遗传特征的单个CpG位点甲基化水平，提出了一种发现lncRNA的新方法。
结果
使用该方法，发现了一种名为LINC02892的新型反义lncRNA，它呈现三种转录本，无编码蛋白质的能力，且分布于细胞核、细胞质和外泌体中。
结论
当前的发现策略可用于鉴定受甲基化异常影响的新型非编码RNA。
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