College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, China.
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, China.
Poult Sci. 2022 Dec;101(12):102231. doi: 10.1016/j.psj.2022.102231. Epub 2022 Oct 8.
Fasting-refeeding perturbation has been extensively used to reveal specific genes and metabolic pathways that control energy metabolism in chickens. In this study, 200 chickens were randomly assigned to 2 groups after hatching: the control group (C, fed ad libitum) and the fasting-refeeding group (T, water ad libitum). The chicks in Group T were fasted for 72 h, and then fed for another 48 h. Liver, hypothalamus, and adipose samples were collected at 0 (F0), 24 (F24), 48 (F48), and 72 h (F72) after fasting and 4 (FR4), 12 (FR12), 24 (FR24), and 48 h (FR48) after refeeding, respectively. Results showed that Group T had a significantly higher number of liver vacuoles (P < 0.05 or P < 0.01) and a significantly lower gray value of Sudan IIIstained sections (P < 0.05 or P < 0.01) than Group C at F48-FR48. In addition, compared with the Group C, fasting and refeeding reduced the expression of stearoyl CoA desaturase (SCD) mRNA (P < 0.05 or P < 0.01) in the liver and adipose tissues, the expression of glucocorticoid receptor (GR) mRNA (P < 0.05 or P < 0.01) in the liver, adipose, and hypothalamus tissues, and the expression of fatty acid synthase (FAS) mRNA (P < 0.05 or P < 0.01) in the liver at F24-FR24. Moreover, relative to those in Group C, fasting and refeeding increased the mRNA expression levels of adenosine monophosphate-activated protein kinase (AMPK) α, AMPKβ, and AMPKγ in the hypothalamus (P < 0.05 or P < 0.01) at F24-FR24. In conclusion, fasting and refeeding increased the fat content of the liver, and the expression of lipolytic genes in the hypothalamus (e.g., AMPKα, AMPKβ, and AMPKγ) but decreased the expression of fat synthesis genes in the liver (e.g., SCD, GR, and FAS), adipose (SCD and GR), and hypothalamus (GR).
禁食-再喂养干扰已被广泛用于揭示控制鸡能量代谢的特定基因和代谢途径。在这项研究中，孵化后将 200 只鸡随机分为 2 组：对照组（C，自由采食）和禁食-再喂养组（T，自由饮水）。组 T 的小鸡禁食 72 h，然后再喂养 48 h。分别在禁食 0（F0）、24（F24）、48（F48）和 72 h（F72）以及再喂养 4（FR4）、12（FR12）、24（FR24）和 48 h（FR48）后采集肝脏、下丘脑和脂肪组织样本。结果表明，与 C 组相比，在 F48-FR48 时，T 组的肝脏空泡数明显增多（P < 0.05 或 P < 0.01），苏丹 III 染色切片的灰度值明显降低（P < 0.05 或 P < 0.01）。此外，与 C 组相比，禁食和再喂养降低了肝脏和脂肪组织中硬脂酰辅酶 A 去饱和酶（SCD）mRNA 的表达（P < 0.05 或 P < 0.01），肝脏、脂肪和下丘脑组织中糖皮质激素受体（GR）mRNA 的表达（P < 0.05 或 P < 0.01），以及肝脏中脂肪酸合酶（FAS）mRNA 的表达（P < 0.05 或 P < 0.01）在 F24-FR24 时。此外，与 C 组相比，在 F24-FR24 时，下丘脑的腺苷酸活化蛋白激酶（AMPK）α、AMPKβ 和 AMPKγ mRNA 表达水平升高（P < 0.05 或 P < 0.01）。综上所述，禁食-再喂养增加了肝脏的脂肪含量，以及下丘脑（如 AMPKα、AMPKβ 和 AMPKγ）中脂肪分解基因的表达，但降低了肝脏（如 SCD、GR 和 FAS）、脂肪（SCD 和 GR）和下丘脑（GR）中脂肪合成基因的表达。
