Bae Jiyun, Jin Sangrak, Kang Seulgi, Cho Byung-Kwan, Oh Min-Kyu
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea; KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea.
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea; KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea.
Curr Opin Biotechnol. 2022 Dec;78:102836. doi: 10.1016/j.copbio.2022.102836. Epub 2022 Nov 2.
The global climate crisis has led to the transition toward the sustainable production of chemicals and fuels with a low carbon footprint. Microbial utilization of one-carbon (C1) substrates, such as carbon dioxide, carbon monoxide, methane, formate, and methanol, may be a promising replacement for the current fossil fuel-based industry. However, natural C1-utilizing microbes are currently unsuitable for industrial applications because of their slow growth and low carbon conversion efficiency, which results in low productivity and yield. Here, we review the recent achievements in engineering C1-utilizing microbes with improved carbon assimilation efficiency and describe the development of synthetic microorganisms by introducing natural C1 assimilation pathways in non-C1-utilizing microbes. Finally, we outline the future directions for realizing the industrial potential of C1-utilizing microbes.
全球气候危机已促使向具有低碳足迹的化学品和燃料可持续生产转型。微生物对一碳（C1）底物（如二氧化碳、一氧化碳、甲烷、甲酸盐和甲醇）的利用，可能是当前基于化石燃料的产业的一个有前景的替代方案。然而，目前天然的C1利用微生物由于生长缓慢和碳转化效率低，不适用于工业应用，这导致生产率和产量较低。在此，我们综述了在工程改造C1利用微生物以提高碳同化效率方面的最新成果，并描述了通过在非C1利用微生物中引入天然C1同化途径来开发合成微生物的情况。最后，我们概述了实现C1利用微生物工业潜力的未来方向。
