School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu, China.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu, China.
Bioresour Technol. 2023 Jan;367:128251. doi: 10.1016/j.biortech.2022.128251. Epub 2022 Nov 2.
d-Arabitol is a top value-added compound with wide applications in the food, pharmaceutical and biochemical industries. Nevertheless, sustainable biosynthesis of d-arabitol is limited by lack of efficient strains and suitable fermentation process. Herein, metabolic engineering and process optimization were performed in Zygosaccharomyces rouxii to overcoming these limitations. Adopting systems metabolic engineering include enhancement of innate biosynthetic pathway, supply of precursor substrate d-ribulose-5P and cofactors regeneration, a novel recombinant strain ZR-5A with good performance was obtained, which boosted d-arabitol production up to 29.01 g/L, 59.31 % higher than the parent strain. Further with the optimum medium composition and fed-batch fermentation, the strain ZR-5A finally produced 149.10 g/L d-arabitol with the productivity of 1.04 g/L/h, which was the highest titer ever reported by Z.rouxii system. This is the first report on the use of metabolic engineering to construct Z. rouxii chassis for the sustainable production of d-arabitol.
d-阿拉伯糖醇是一种高附加值的化合物，在食品、制药和生化等行业有着广泛的应用。然而，d-阿拉伯糖醇的可持续生物合成受到缺乏高效菌株和合适发酵工艺的限制。在此，通过代谢工程和过程优化在鲁氏酵母（Zygosaccharomyces rouxii）中克服了这些限制。采用系统代谢工程，包括增强内源性生物合成途径、提供前体底物 d-核酮糖-5-磷酸和辅助因子再生，获得了具有良好性能的新型重组菌株 ZR-5A，其 d-阿拉伯糖醇产量高达 29.01 g/L，比原始菌株提高了 59.31%。进一步优化培养基组成和补料分批发酵，菌株 ZR-5A 最终生产 149.10 g/L 的 d-阿拉伯糖醇，得率为 1.04 g/L/h，这是鲁氏酵母系统报道的最高产量。这是首次利用代谢工程构建鲁氏酵母底盘，用于可持续生产 d-阿拉伯糖醇的报道。
