Department of Biotechnology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal 576104, India.
Department of Cell and Molecular Biology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal 576104, India.
Environ Toxicol Pharmacol. 2022 Nov;96:104010. doi: 10.1016/j.etap.2022.104010. Epub 2022 Nov 2.
Bisphenol A (BPA) mimics estrogen and consequently suspected to be detrimental to female reproductive system. Biomonitoring confirms the BPA burden in body leading to a complex condition called polycystic ovarian syndrome (PCOS) which is frequently attributed to female infertility. Due to unclear precise molecular pathomechanisms of BPA in PCOS, we intend to examine the molecular mechanisms of the reproductive, endocrine, mitochondrial features, and cellular senescence in BPA-treated rats. We analyzed vaginal smears and ovarian follicles using microscope, assessed sex hormones by ELISA, analyzed BPA target gene expression by semi-quantitative RT-PCR, assessed senescence induction by β-galactosidase staining and immunofluorescence in BPA-treated rats. Our data showed hormonal imbalance, impaired folliculogenesis, abnormal expression patterns of target genes, CDKN2A overexpression and enhanced ROS levels in BPA-treated rats. This study provides insights on the effects of BPA exposure on ovulatory, hormonal, mitochondrial dysfunction, and senescence that benefit in better understanding of PCOS induced by BPA.
双酚 A（BPA）模拟雌激素，因此被怀疑对女性生殖系统有害。生物监测证实了体内的 BPA 负担，导致一种称为多囊卵巢综合征（PCOS）的复杂情况，这通常归因于女性不孕。由于 BPA 在 PCOS 中的精确分子发病机制尚不清楚，我们旨在研究 BPA 处理大鼠的生殖、内分泌、线粒体特征和细胞衰老的分子机制。我们使用显微镜分析阴道涂片和卵巢卵泡，通过 ELISA 分析性激素，通过半定量 RT-PCR 分析 BPA 靶基因表达，通过β-半乳糖苷酶染色和免疫荧光评估 BPA 处理大鼠的衰老诱导。我们的数据显示，BPA 处理大鼠存在激素失衡、卵泡发生受损、靶基因表达模式异常、CDKN2A 过表达和 ROS 水平升高。这项研究提供了关于 BPA 暴露对排卵、激素、线粒体功能障碍和衰老的影响的见解，有助于更好地理解 BPA 引起的 PCOS。
